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-   -   MTT and alamar blue assay sensitivity and Mycobacterium tuberculosis (http://www.molecularstation.com/forum/microbiology-forum/84070-mtt-alamar-blue-assay-sensitivity-mycobacterium-tuberculosis.html)

charissa 11-17-2011 04:07 PM

MTT and alamar blue assay sensitivity and Mycobacterium tuberculosis
 
I have noticed that the MTT growth curves for Mtb strains become less linear after the cultures have been allowed to incubate (while being agitated in a shaking incubator) for more than 15 days. This does not appear to be a problem with the Alamar Blue reagent. Could this be due to the increased concentration of cells in the broth or a result of the cells moving into the stationary phase? please help. thank you

butters 11-18-2011 05:10 AM

Re: MTT and alamar blue assay sensitivity and Mycobacterium tuberculosis
 
uhm... your culturing mycobacterium in flask?! I am just curious what culture medium did you use?

Jorge1907 11-23-2011 12:09 PM

Re: MTT and alamar blue assay sensitivity and Mycobacterium tuberculosis
 
what is the measure of growth that shows linearity?

charissa 12-08-2011 08:28 AM

Re: MTT and alamar blue assay sensitivity and Mycobacterium tuberculosis
 
I am growing the cultures using 7H9 medium.
Growth curves: measure of absorbance at 600 nm versus interval (e.g Day 0, etc.)

osama al refa'e 03-16-2012 06:09 PM

Re: MTT and alamar blue assay sensitivity and Mycobacterium tuberculosis
 
What if you didnt use shaking incubater maybe this will help


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