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| Hi what is the range of overexpression of protein in E.coli (mg/liter of culture) in soluble form and also in inclusion bodies form. did anyone has studied it or overexpress the protein itself? regards aftab |
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| Hey there Aftab, with E.coli I have read that proteins can be overexpressed at rates from 4. 4 mg/L to even 60 mg/L or more. some references: http://cat.inist.fr/?aModele=afficheN&cpsidt=15884266 http://cat.inist.fr/?aModele=afficheN&cpsidt=13908297 http://www.springerlink.com/index/N3742V57N350P75N.pdf |