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Need help with basic things (parasite/microscope)

Need help with basic things (parasite/microscope) - Microbiology Forum

Need help with basic things (parasite/microscope) - Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum.

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Old 06-28-2010, 09:37 AM
Pipette Filler
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Default Need help with basic things (parasite/microscope)

Hi: I hope that it is ok to post such basic questions, and i hope that someone will provide me with guidance.

I am looking for help in a protozoa issue. I am not a scientist but have some basic understanding of microscopy. I have suffered with a protozoa parasite that has been confirmed by PCR testing, although its taxonomy is unknown. I personally think that it is blastocystis hominis (genotype). The medical system has been unhelpful to me and it is up to me to research this myself. I have also had an amatuer researcher send me pictures of the parasite, and again it appears to be blastocystis hominis.

Unfortunately the research of blastocystis is poor, Wiki states:

Research on Blastocystis hominis is limited, with large gaps remaining in our understanding of its life cycle, transmission mechanisms, incubation period, epidemiology, and treatment options

The main issue is that treatment protocols are not well established and it is my thinking that the organism can develop resistance to drugs, and it is well known that a multiple drug approach is necessary. There are a number of pharma agents than CAN be effective.

I own an older medical microscope (AO Spencer) that has resolution up to 1000X. I was hoping to utilize the scope in some protocols. At first I was thinking of attempting to culture but in some preliminary research, it appears that this is a process that might be above me. In additon to this, I am scared of the pathogenic nature of this organism (I have been quite ill) and my inability to keep it in its place. It was my hope that thru culturing I could introduce aqueous extracts of either pharma or herbal solutions to test effectiveness.

My second idea revolves around looking at random stool samples. I was thinking that if I could have a standarized sample that I could utilize a simple count of the organisms in said sample and know whether reduction is occuring as I introduce pharma agents. I could also view to see if apotosis is occuring. Then I have toyed with the idea of introducing aqueous solutions into the slide to see if reactions occur.

Does any ot this sound logical or is there any better ways? I appreciate any responses!
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