Go Back   Molecular Biology Forum > Molecular Research Topics Forum > Animal and Molecular Model Systems > Microbiology Forum
Register Blogs FAQ Members List Calendar Science Groups New! Arcade Search Today's Posts Mark Forums Read

Microbiology Forum Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum.


bacteria Videos
dna-electroporation-ligation
dna-electroporation-ligation DNA Electroporation with subsequent transformation of bacteria.


IPTG induction

Microbiology Forum

Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum.



Register Molecular Biology Forums
Reply
 
LinkBack Thread Tools Display Modes
  #1 (permalink)  
Old 12-06-2006, 01:41 PM
M.D/Ph.D
Points: 6,097, Level: 53Points: 6,097, Level: 53Points: 6,097, Level: 53
Activity: 11%Activity: 11%Activity: 11%
 

Join Date: Jun 2006
Location: Pakistan
Posts: 439
aftabac RSS Feed
Send a message via MSN to aftabac Send a message via Yahoo to aftabac
Default IPTG induction

Hi
for the induction of expression of our gene of interest that is based on lac promoter we usually use IPTG instead of lactose, can anyone of you would like to explain it why we preferably use IPTG instead of Lactose.

regards
aftab
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!Spurl this Post!Reddit!
Reply With Quote
Alt Today
Advertising
Google Adsense
 
This advertising will not be shown
in this way to registered members.
Register your free account today
and become a member on
Molecular Biology Forum
Standard Sponsored Links

  #2 (permalink)  
Old 12-06-2006, 02:15 PM
Graduate Student
Points: 1,844, Level: 26Points: 1,844, Level: 26Points: 1,844, Level: 26
Activity: 5%Activity: 5%Activity: 5%
 

Join Date: Aug 2006
Location: Ithaca, NY
Posts: 128
kmunson779 RSS Feed
Default Re: IPTG induction

[Only registered users see links. ]

Quote:
Unlike allolactose, the sulfur (S) atom creates a chemical bond which is non-hydrolyzable by the cell, preventing the cell from "eating up" or degrading the inductant.
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!Spurl this Post!Reddit!
Reply With Quote
  #3 (permalink)  
Old 10-05-2007, 06:12 AM
Pipette Filler
Points: 377, Level: 7Points: 377, Level: 7Points: 377, Level: 7
Activity: 0%Activity: 0%Activity: 0%
 

Join Date: Oct 2007
Posts: 1
mani14s RSS Feed
Default Re: IPTG induction

IPTG is toxic to cells & also costly. so its better to go for lactose
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!Spurl this Post!Reddit!
Reply With Quote
  #4 (permalink)  
Old 10-06-2007, 08:49 AM
M.D/Ph.D
Points: 6,097, Level: 53Points: 6,097, Level: 53Points: 6,097, Level: 53
Activity: 11%Activity: 11%Activity: 11%
 

Join Date: Jun 2006
Location: Pakistan
Posts: 439
aftabac RSS Feed
Send a message via MSN to aftabac Send a message via Yahoo to aftabac
Default Re: IPTG induction

yes, IPTG is toxic to cell but its only toxic when its concentration is high. but the important thing is that it is not hyrolyzed by the cells, so it stayes inside the cells and give a constant induction and expression of the gene and hence protein, but as compared to it if lactose is used it is metabolized and so no more lactose is there in the cells that could induce the expression of gene.
regards
aftab
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!Spurl this Post!Reddit!
Reply With Quote
  #5 (permalink)  
Old 10-01-2008, 01:26 AM
Pipette Filler
Points: 12, Level: 1Points: 12, Level: 1Points: 12, Level: 1
Activity: 5%Activity: 5%Activity: 5%
 

Join Date: Oct 2008
Posts: 1
one.tjc RSS Feed
Question Basic question

Sorry to drag up an old thread, but when IPTG (0.1M) is added to a fermentation culture, what effects does it have on the growth rate of the cells.

I'm assuming that the cells will stop dividing and instead churn out protein, so the A600 will level off as this occurs?

Cheers!
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!Spurl this Post!Reddit!
Reply With Quote
Reply

Tags
induction , iptg

Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On
Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
tumor induction -query bhunni Molecular Biology Techniques 2 04-18-2008 05:49 PM
Inducing Protein Expression IPTG vs Lactose deebee12 Protein Science 2 10-01-2007 12:23 PM
preference in IPTG trademark? dave_theater Lab Equipment Discussion and Reviews 0 03-28-2007 03:51 PM


All times are GMT. The time now is 09:17 AM.


Powered by vBulletin® Version 3.7.1
Copyright ©2000 - 2008, Jelsoft Enterprises Ltd.
Copyright 2005-2007 Molecular Station | All Rights Reserved