I´m doing some inhibitory tests on an S. epidermidis
strain, with different P. acnes
strains as indicator strains.
When i inoculate the S. epidermidis
strain onto a sterile filter placed on an agar plate, there is no problem in removing the filter and bacteria and swim the plate with the indicator strain.
However, I need a good advice when inoculating the S. epidermidis
directly onto the agar plate (just a quick "stab" in the center of the plate, so that one big coclony appears after incubation), on how to remove the colony without smearing it on the plate. I tried with sterile water and micro glass plates without success. And even when I treat with chloroform afterwards it is still not succesfull. I need to get a clear, round inhibition zone, just as I get when they have been grown on filters prior to the swimming with the indicator strains.
I hope that any of you have any suggestions on what to do.