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removal of a bacterial colony from an agar plate

removal of a bacterial colony from an agar plate - Microbiology Forum

removal of a bacterial colony from an agar plate - Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum.


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Old 09-24-2009, 08:29 AM
Pipette Filler
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Default removal of a bacterial colony from an agar plate



Im doing some inhibitory tests on an S. epidermidis strain, with different P. acnes strains as indicator strains.
When i inoculate the S. epidermidis strain onto a sterile filter placed on an agar plate, there is no problem in removing the filter and bacteria and swim the plate with the indicator strain.
However, I need a good advice when inoculating the S. epidermidis directly onto the agar plate (just a quick "stab" in the center of the plate, so that one big coclony appears after incubation), on how to remove the colony without smearing it on the plate. I tried with sterile water and micro glass plates without success. And even when I treat with chloroform afterwards it is still not succesfull. I need to get a clear, round inhibition zone, just as I get when they have been grown on filters prior to the swimming with the indicator strains.
I hope that any of you have any suggestions on what to do.
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