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Microbio Digest, Vol 49, Issue 2

Microbio Digest, Vol 49, Issue 2 - Microbiology Forum

Microbio Digest, Vol 49, Issue 2 - Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum.


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  #1  
Old 06-18-2009, 05:26 PM
Melissa A. Barlett
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Default Microbio Digest, Vol 49, Issue 2




I typically use kits for DNA extraction, with some additions, and have
done well with difficult sediment samples. The two kits I tend to use
are the Bio101 FastDNA SPIN kit for soil and the MoBio PowerSoil kit. In
addition to these kits, I have on occasion added a heating step at 70C
for 1 hour at the beginning while the cells are in the detergent buffer.
Plant kits can also be good for removing other interfering substances.

I have also had the problem where I got DNA, but not so much that it was
easy to tell, however, the subsequent PCR was blocked by an interferent
of some kind, making me think my extraction was bad. If you think this
might be your problem, the Promega Wizard DNA clean-up kit is great for
making bad DNA usable.

Melissa
University of Massachusetts


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  #2  
Old 06-19-2009, 06:49 AM
lokesh jain
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Default Microbio Digest, Vol 49, Issue 2

On Jun 18, 10:26*pm, "Melissa A. Barlett"
<[Only registered users see links. ]> wrote:

hi
here is lokesh jain from india
this is my first visit at this group, have done pG in microbiology.
keep in touch


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  #3  
Old 06-24-2009, 03:58 PM
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Default Re: Microbio Digest, Vol 49, Issue 2

yea, have used the promega kit before and it works well, how did your controls perform? Did they show a chance for inhibition?
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