Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Animal and Molecular Model Systems > Microbiology Forum
Register Search Today's Posts Mark Forums Read

Microbiology Forum Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum.


E.coli MC4100 thi- genotype

E.coli MC4100 thi- genotype - Microbiology Forum

E.coli MC4100 thi- genotype - Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 02-24-2009, 04:09 AM
Julian Spagnuolo
Guest
 
Posts: n/a
Default E.coli MC4100 thi- genotype




Hello fellow slaves of science.
I'm trying to create a pspA reporter plasmid by linking the psp
promoter using the thi- genotype of E.coli MC4100 as an auxotrophic
marker, however I'm having 'a little' trouble finding out which gene
has actually been deleted or mutated so that I can incorporate it into
my plasmid.
Does anyone out there know what gene the thi- in E.coli MC4100 actually is?
Thanks,
Julian

__________________________________________________ _______________
Got a 2008 financial hangover? Find a cure at MSN NZ Money
[Only registered users see links. ]
Reply With Quote
  #2  
Old 03-09-2009, 01:25 AM
N10
Guest
 
Posts: n/a
Default E.coli MC4100 thi- genotype


"Julian Spagnuolo" <[Only registered users see links. ]> wrote in message
news:[Only registered users see links. ] .net...

Hello fellow slaves of science.
I'm trying to create a pspA reporter plasmid by linking the psp
promoter using the thi- genotype of E.coli MC4100 as an auxotrophic
marker, however I'm having 'a little' trouble finding out which gene
has actually been deleted or mutated so that I can incorporate it into
my plasmid.
Does anyone out there know what gene the thi- in E.coli MC4100 actually is?
Thanks,
Julian


HI

Does this help

[Only registered users see links. ]


Best N10

__________________________________________________ _______________
Got a 2008 financial hangover? Find a cure at MSN NZ Money
[Only registered users see links. ]


Reply With Quote
  #3  
Old 03-09-2009, 08:49 PM
Tom Knight
Guest
 
Posts: n/a
Default E.coli MC4100 thi- genotype

> "Julian Spagnuolo" <[Only registered users see links. ]> wrote in message

You might check the E. coli genetic stock center listings:
[Only registered users see links. ]

They don't list this strain as thi-, but it could be part of the three
deletion regions.
Reply With Quote
  #4  
Old 03-25-2009, 07:53 PM
David F. Spencer
Guest
 
Posts: n/a
Default E.coli MC4100 thi- genotype

Julian Spagnuolo wrote:

I don't mean to appear unkind but I assume you have a reason to believe this
strain really is a thiamine requirer? (It is true that many K-12 strains are).
I have done some searching to trace the pedigree of MC4100 but I can find no
reference to its being thi- (nor in fact for any of its ancestors).
MC4100 is a derivative of MG1655 which is in turn a fairly recent derivative
of W1485, a strain very close to the original K-12. It is through the W1485
derivative W2637 that W3110 was produced (W3110 being a fairly well-known
K-12). Some of this is detailed in Barbara Bachmann's classic 1972
Bacteriological Reviews paper 'Pedigrees of Some Mutant Strains of Escherichia
coli K-12'.
I have never seen a genotype for MC4100 that mentions 'thi', and none of the
ancestors of MC4100 are acknowledged to have a mutation in any thiamine
synthesis or related gene(s).
Reply With Quote
  #5  
Old 04-15-2010, 07:52 PM
Pipette Filler
Points: 2, Level: 1 Points: 2, Level: 1 Points: 2, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Apr 2010
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: E.coli MC4100 thi- genotype

Quote:
Originally Posted by David F. Spencer View Post
Julian Spagnuolo wrote:

I don't mean to appear unkind but I assume you have a reason to believe this
strain really is a thiamine requirer? (It is true that many K-12 strains are).
I have done some searching to trace the pedigree of MC4100 but I can find no
reference to its being thi- (nor in fact for any of its ancestors).
MC4100 is a derivative of MG1655 which is in turn a fairly recent derivative
of W1485, a strain very close to the original K-12. It is through the W1485
derivative W2637 that W3110 was produced (W3110 being a fairly well-known
K-12). Some of this is detailed in Barbara Bachmann's classic 1972
Bacteriological Reviews paper 'Pedigrees of Some Mutant Strains of Escherichia
coli K-12'.
I have never seen a genotype for MC4100 that mentions 'thi', and none of the
ancestors of MC4100 are acknowledged to have a mutation in any thiamine
synthesis or related gene(s).
I found this post via Google for "MC4100 pedigree" and I must interject to say that David Spencer is incorrect on several counts.
  • According to the introduction in Ferenci et al, MC4100 is not derived from MG1655.
  • The oldest reference to MC4100 at the CGSC website is to Casadaban 1976, where MC4100 is in the strain table, and thi is listed in its genotype.
  • MC4100 does not appear in the strains listed in Bachmann's derivation of mutant strain papers.
I had links to the papers, but as a new user I can't post URLs
Reply With Quote
Reply

Tags
ecoli , genotype , mc4100 , thi


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
At gene expression in E.coli Jiang Yakim Arabidopsis and Plant Biology 0 10-10-2008 04:42 AM
Protein secretion in E.coli Duke Protein Science 3 01-24-2008 09:11 PM
Hof fast can E.Coli metabolize hexoses? Roberto Carlos Microbiology Forum 2 08-07-2006 06:45 AM
Brown E.coli Trond Erik Vee Aune Microbiology Forum 3 06-14-2005 12:08 PM
HA tagging on E.coli chromosome tracy Microbiology Forum 0 06-29-2004 07:10 PM


All times are GMT. The time now is 09:41 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.14337 seconds with 16 queries