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11-11-2006, 07:38 AM
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 Protocol for Long-term storage of Bacterial Stock Can someone provide the protocol for Long-term storage of Bacterial Stocks. thanks         |
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11-13-2006, 04:32 PM
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 Re: Protocol for Long-term storage of Bacterial Stock It's in Molecular Cloning. Add 0.5 mL autoclaved 60% glycerol to 1.5 mL overnight bacterial culture in a screw-cap tube. Vortex to mix & label the tube. Quick-freeze in liquid nitrogen. Store at -80. To recover the culture, scrape the frozen surface of the culture with a sterile pipet tip and streak on a selective agar plate. Keep the storage tube FROZEN at All Times. Incubate the plate at 37 degrees per usual. |
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