Originally Posted by gatrellm
I am trying to teach high school biology in a school with a serious lack of equipment. Can I sterilize nutrient agar without one? Any suggestions
A few pointers when working without an autoclave -
1. Get the students to work under flame and close the windows.
2. Don't use a pressure cooker instead as they are pretty dangerous to use.
3. Nutrient agar is almost always contaminated through handling
e.g. opening lids, dirty bottles, sniffing it, whatever else high school students do...
P.S. I'd say just mix it up fresh and run it to the kid's benches like it's a tray of hot brownies. They won't know the difference
P.P.S. Use autoclave tape but borrow a brown texta from the art dept.