Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Animal and Molecular Model Systems > Microbiology Forum
Register Search Today's Posts Mark Forums Read

Microbiology Forum Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum.


Autoclave Help

Autoclave Help - Microbiology Forum

Autoclave Help - Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 01-28-2009, 10:30 PM
Pipette Filler
Points: 3, Level: 1 Points: 3, Level: 1 Points: 3, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2009
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Smile Autoclave Help



I am trying to teach high school biology in a school with a serious lack of equipment. Can I sterilize nutrient agar without one? Any suggestions

Gatrellm
Reply With Quote
  #2  
Old 01-29-2009, 11:22 AM
Ph.D Doctorate
Points: 3,253, Level: 37 Points: 3,253, Level: 37 Points: 3,253, Level: 37
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jun 2008
Posts: 154
Thanks: 0
Thanked 17 Times in 16 Posts
Default Re: Autoclave Help

You can try a kitchen pressure cooker and there's always Tyndalization for broth.


Maybe you could contact some local testing labs and beg media that's expired.
Reply With Quote
  #3  
Old 01-29-2009, 03:54 PM
Graduate Student
Points: 2,923, Level: 35 Points: 2,923, Level: 35 Points: 2,923, Level: 35
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Aug 2006
Location: Ithaca, NY
Posts: 145
Thanks: 0
Thanked 17 Times in 12 Posts
Default Re: Autoclave Help

I read an interesting [Only registered users see links. ] on microwave sterilization a few months back.
Reply With Quote
  #4  
Old 01-29-2009, 04:18 PM
Ph.D Doctorate
Points: 3,253, Level: 37 Points: 3,253, Level: 37 Points: 3,253, Level: 37
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jun 2008
Posts: 154
Thanks: 0
Thanked 17 Times in 16 Posts
Default Re: Autoclave Help

Microwave is not used for media sterilization - more so for melting agar. Still - maybe you could try it - what do you have to lose?
Reply With Quote
  #5  
Old 01-29-2009, 04:35 PM
Aga's Avatar
Aga Aga is offline
Post-Doc
Points: 2,239, Level: 30 Points: 2,239, Level: 30 Points: 2,239, Level: 30
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2008
Posts: 270
Thanks: 25
Thanked 78 Times in 64 Posts
Default Re: Autoclave Help

You can make some kind of water bath for tyndalization and pressure cooker can be useful for sterilization.

Using microwave oven you'll get your agar melted but any liquid evaporates quite quickly and I don't know if you can close your tubes or flasks tight using microwave, so you'll finally get a lot more concentrated medium. I don't recommend microwaving.
Reply With Quote
The Following User Says Thank You to Aga For This Useful Post:
admin (01-31-2009)
  #6  
Old 01-29-2009, 11:53 PM
Ph.D Doctorate
Points: 3,253, Level: 37 Points: 3,253, Level: 37 Points: 3,253, Level: 37
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jun 2008
Posts: 154
Thanks: 0
Thanked 17 Times in 16 Posts
Default Re: Autoclave Help

Tyndalization does not work for agar media.
Reply With Quote
  #7  
Old 01-30-2009, 08:40 AM
Aga's Avatar
Aga Aga is offline
Post-Doc
Points: 2,239, Level: 30 Points: 2,239, Level: 30 Points: 2,239, Level: 30
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2008
Posts: 270
Thanks: 25
Thanked 78 Times in 64 Posts
Default Re: Autoclave Help

Quote:
Originally Posted by Jorge1907 View Post
Tyndalization does not work for agar media.
Some media are not designed for sterilization. Slanetz and Bartley agar base is one example - you can't sterilize it and the only thing you must do is to slowly bring it to boil untill agar is dissolved (off course it concernes pulverized medium ready to made).

In this case you don't even use tyndalization. Agar will dissolve, I checked it.
Reply With Quote
  #8  
Old 01-30-2009, 11:00 AM
Ph.D Doctorate
Points: 3,253, Level: 37 Points: 3,253, Level: 37 Points: 3,253, Level: 37
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jun 2008
Posts: 154
Thanks: 0
Thanked 17 Times in 16 Posts
Default Re: Autoclave Help

This discussion involved nutrient agar for school demonstration. A highly selective, differential medium such as Slanetz and Bartley is not relevant.
Reply With Quote
  #9  
Old 01-30-2009, 01:54 PM
NATHANALANPATRICKROGERS's Avatar
Summer Student
Points: 306, Level: 6 Points: 306, Level: 6 Points: 306, Level: 6
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2009
Location: Maroochydore
Posts: 73
Thanks: 4
Thanked 13 Times in 11 Posts
Default Re: Autoclave Help

Quote:
Originally Posted by gatrellm View Post
I am trying to teach high school biology in a school with a serious lack of equipment. Can I sterilize nutrient agar without one? Any suggestions

Gatrellm
A few pointers when working without an autoclave -

1. Get the students to work under flame and close the windows.
2. Don't use a pressure cooker instead as they are pretty dangerous to use.
3. Nutrient agar is almost always contaminated through handling
e.g. opening lids, dirty bottles, sniffing it, whatever else high school students do...

P.S. I'd say just mix it up fresh and run it to the kid's benches like it's a tray of hot brownies. They won't know the difference

P.P.S. Use autoclave tape but borrow a brown texta from the art dept.
Reply With Quote
The Following User Says Thank You to NATHANALANPATRICKROGERS For This Useful Post:
admin (01-31-2009)
  #10  
Old 01-30-2009, 10:18 PM
Aga's Avatar
Aga Aga is offline
Post-Doc
Points: 2,239, Level: 30 Points: 2,239, Level: 30 Points: 2,239, Level: 30
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2008
Posts: 270
Thanks: 25
Thanked 78 Times in 64 Posts
Default Re: Autoclave Help

Quote:
Originally Posted by Jorge1907 View Post
This discussion involved nutrient agar for school demonstration. A highly selective, differential medium such as Slanetz and Bartley is not relevant.
The discussion started in the first post - yes, but maybe nutrient agar was just an example to address the problem? And who knows who will read these posts?

I guess all ideas are relevant as far as they are connected to replace autoclave with sth else.
And once more - you'll get agar dissolved by tyndalization or boiling. You won't have it sterile, that's for sure.
Reply With Quote
Reply

Tags
autoclave


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Contamination of 7H11 agar plates martin rao Microbiology Forum 4 10-31-2010 08:39 PM
Autoclave Bag johan.apryanto Lab Equipment Discussion and Reviews 0 01-23-2009 08:38 AM
Bench top autoclave ebrenner Lab Equipment Discussion and Reviews 1 01-23-2009 08:10 AM
About TE and Tris-HCl sterilize with autoclave fabio Protocols and Methods Forum 8 11-07-2004 06:29 PM
Bacterial contamination- need some help Aaron Hicks Microbiology Forum 4 08-07-2003 08:55 AM


All times are GMT. The time now is 01:05 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.16831 seconds with 16 queries