Autoclave Help

[Jorge1907]

Nutrient agar was what was SAID. They don't even have an autoclave - think they have your exceedingly obscure medium? You are so kind.


Edit (admin) please refrain from taking this personally and no insults please

[Aga]

Originally Posted by Jorge1907

Nutrient agar was what was SAID. They don't even have an autoclave - think they have your exceedingly obscure medium? You are so kind.

I just gave an example, take it easy I didn't suppose you could take it so seriously. No offence, I just try to help as you do, and treat this Slanetz thing as an off-top .

It's just that we also teach students at high school/university, and I guess there are plenty of differences between high schools/universities in different countries (not only in syllabus).

[awtoyscom]

Microwave is not used for media sterilization - more so for melting agar. Still - maybe you could try it - what do you have to lose?

[Jorge1907]

You guess - as long as they're your irrelevant ideas? Tell us aga - when have you used tyndalization at all - much less for agar?

[Aga]

No - still you don't understand Jorge1907. Did I said about what I do everyday in my lab? I gave some examples how to overcome autoclave problem. I hope you get it.

And if it comes to that - answer to your question: I use nutrient agar or TSA for my slants (besides freezing my strains I also keep them on slants for shorter period when I need). Firstly I prepare dissolved medium - I just boil it once and agar dissolves (so the rest of the ingredients) - I use water bath usually (we also have Koch sterilizer when I need larger volumes).

Pre-prepared medium in flasks I distribute to tubes and then sterilize properly. The first step of slants preparation - that's an example when I may use boiling for agar. Boiling for agar - yes, tyndalization - rather not, but it was said with reference to replacing sterilization.

When have I used it? Recently? I used it one week ago. On friday to be specific.

And as you mentioned before - this all was supposed to be the basis for high school class where there is no autoclave. Is tyndallization irrelevant there? Supposing they don't have funds for pressure cooker? Just imagine We're just disscussing problem here, that's all.

Cheers Jorge1097 That's actually getting interersting.

[Jorge1907]

Oh sure - a high school teacher with n o autoclave has alot of media. suuree>>>>>>>>

you've never used tyndalization aga - admit it. You have no idea how multiply heatings affect agar, do you?! try to not blow smoke aga - your lie is obvious.

[Aga]

Originally Posted by Jorge1907

Oh sure - a high school teacher with n o autoclave has alot of media. suuree>>>>>>>>

I thought I said Slanetz & Bartley was an example? BTW we get some selective media if they are outdated from veterinary centre so don't be so sure what they have.

Originally Posted by Jorge1907

you've never used tyndalization aga - admit it. You have no idea how multiply heatings affect agar, do you?!

Well yes, I did try to heat agar medium for 3 times - it affected the medium, all right, but it still was usable - at least would be usable in the case of high school teacher who doesn't have an autoclave, and we're not talking about reference analyses but about replacing an autoclave.

And I don't use tyndalization for agar media on everyday basis because I have an autoclave. I thought we tried to find some solutions but so far you negate mine not justifying that. I've never said I know everything but if you know more - why don't you share? It's easy to say "this is irrelevant, it doesn't work". Maybe that's true - justify it.

I also used the term - boiling - look at the post, and you seem to only refer to tyndalization because you find it interesting to pick holes in everything I say.

[MoleculeInAction]

Originally Posted by gatrellm

I am trying to teach high school biology in a school with a serious lack of equipment. Can I sterilize nutrient agar without one? Any suggestions

Gatrellm

If your school lack those expensive autoclave , You can use pressure cooker as the option which also work on the smae principle.

Please try to follow good laboratory practices!

[rockq]

use pressure cooker,we used to use it from our college when we lack space in our autoclave,it really works

[SebaQ]

Originally Posted by Aga

we're not talking about reference analyses but about replacing an autoclave.

I think this is what matters... take it easy, Jorge. It's just a high school class, y'know.

Pressure cooker would work kinda fine in most cases, and it'd be the best solution if you lack the autoclave. Obviously it's not the same, but physical principles are the same.

And about tyndalization... why don't you just liquid media if repeatedly heating agar is not recommended?