Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Animal and Molecular Model Systems > Microbiology Forum
Register Search Today's Posts Mark Forums Read

Microbiology Forum Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum.


Autoclave Help

Autoclave Help - Microbiology Forum

Autoclave Help - Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum.


Reply
 
LinkBack Thread Tools Display Modes
  #11  
Old 01-31-2009, 12:03 AM
Ph.D Doctorate
Points: 3,253, Level: 37 Points: 3,253, Level: 37 Points: 3,253, Level: 37
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jun 2008
Posts: 154
Thanks: 0
Thanked 17 Times in 16 Posts
Default Re: Autoclave Help

Nutrient agar was what was SAID. They don't even have an autoclave - think they have your exceedingly obscure medium? You are so kind.


Edit (admin) please refrain from taking this personally and no insults please

Last edited by admin; 01-31-2009 at 07:37 AM. Reason: warning please no insults everyone is here to learn and discuss including me
Reply With Quote
  #12  
Old 01-31-2009, 10:14 AM
Aga's Avatar
Aga Aga is offline
Post-Doc
Points: 2,239, Level: 30 Points: 2,239, Level: 30 Points: 2,239, Level: 30
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2008
Posts: 270
Thanks: 25
Thanked 78 Times in 64 Posts
Default Re: Autoclave Help

Quote:
Originally Posted by Jorge1907 View Post
Nutrient agar was what was SAID. They don't even have an autoclave - think they have your exceedingly obscure medium? You are so kind.
I just gave an example, take it easy I didn't suppose you could take it so seriously. No offence, I just try to help as you do, and treat this Slanetz thing as an off-top .

It's just that we also teach students at high school/university, and I guess there are plenty of differences between high schools/universities in different countries (not only in syllabus).
Reply With Quote
  #13  
Old 01-31-2009, 04:56 PM
Pipette Filler
Points: 4, Level: 1 Points: 4, Level: 1 Points: 4, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2009
Posts: 2
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Autoclave Help

Microwave is not used for media sterilization - more so for melting agar. Still - maybe you could try it - what do you have to lose?
Reply With Quote
  #14  
Old 01-31-2009, 09:19 PM
Ph.D Doctorate
Points: 3,253, Level: 37 Points: 3,253, Level: 37 Points: 3,253, Level: 37
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jun 2008
Posts: 154
Thanks: 0
Thanked 17 Times in 16 Posts
Default Re: Autoclave Help

You guess - as long as they're your irrelevant ideas? Tell us aga - when have you used tyndalization at all - much less for agar?
Reply With Quote
  #15  
Old 01-31-2009, 10:19 PM
Aga's Avatar
Aga Aga is offline
Post-Doc
Points: 2,239, Level: 30 Points: 2,239, Level: 30 Points: 2,239, Level: 30
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2008
Posts: 270
Thanks: 25
Thanked 78 Times in 64 Posts
Default Re: Autoclave Help

No - still you don't understand Jorge1907. Did I said about what I do everyday in my lab? I gave some examples how to overcome autoclave problem. I hope you get it.

And if it comes to that - answer to your question: I use nutrient agar or TSA for my slants (besides freezing my strains I also keep them on slants for shorter period when I need). Firstly I prepare dissolved medium - I just boil it once and agar dissolves (so the rest of the ingredients) - I use water bath usually (we also have Koch sterilizer when I need larger volumes).

Pre-prepared medium in flasks I distribute to tubes and then sterilize properly. The first step of slants preparation - that's an example when I may use boiling for agar. Boiling for agar - yes, tyndalization - rather not, but it was said with reference to replacing sterilization.

When have I used it? Recently? I used it one week ago. On friday to be specific.

And as you mentioned before - this all was supposed to be the basis for high school class where there is no autoclave. Is tyndallization irrelevant there? Supposing they don't have funds for pressure cooker? Just imagine We're just disscussing problem here, that's all.

Cheers Jorge1097 That's actually getting interersting.

Last edited by Aga; 01-31-2009 at 10:28 PM.
Reply With Quote
  #16  
Old 02-01-2009, 12:30 AM
Ph.D Doctorate
Points: 3,253, Level: 37 Points: 3,253, Level: 37 Points: 3,253, Level: 37
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jun 2008
Posts: 154
Thanks: 0
Thanked 17 Times in 16 Posts
Default Re: Autoclave Help

Oh sure - a high school teacher with n o autoclave has alot of media. suuree>>>>>>>>

you've never used tyndalization aga - admit it. You have no idea how multiply heatings affect agar, do you?! try to not blow smoke aga - your lie is obvious.
Reply With Quote
  #17  
Old 02-01-2009, 08:28 AM
Aga's Avatar
Aga Aga is offline
Post-Doc
Points: 2,239, Level: 30 Points: 2,239, Level: 30 Points: 2,239, Level: 30
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2008
Posts: 270
Thanks: 25
Thanked 78 Times in 64 Posts
Default Re: Autoclave Help

Quote:
Originally Posted by Jorge1907 View Post
Oh sure - a high school teacher with n o autoclave has alot of media. suuree>>>>>>>>
I thought I said Slanetz & Bartley was an example? BTW we get some selective media if they are outdated from veterinary centre so don't be so sure what they have.
Quote:
Originally Posted by Jorge1907 View Post
you've never used tyndalization aga - admit it. You have no idea how multiply heatings affect agar, do you?!
Well yes, I did try to heat agar medium for 3 times - it affected the medium, all right, but it still was usable - at least would be usable in the case of high school teacher who doesn't have an autoclave, and we're not talking about reference analyses but about replacing an autoclave.

And I don't use tyndalization for agar media on everyday basis because I have an autoclave. I thought we tried to find some solutions but so far you negate mine not justifying that. I've never said I know everything but if you know more - why don't you share? It's easy to say "this is irrelevant, it doesn't work". Maybe that's true - justify it.

I also used the term - boiling - look at the post, and you seem to only refer to tyndalization because you find it interesting to pick holes in everything I say.

Last edited by Aga; 02-01-2009 at 08:39 AM.
Reply With Quote
  #18  
Old 02-03-2009, 09:35 AM
MoleculeInAction's Avatar
Pipette Filler
Points: 165, Level: 3 Points: 165, Level: 3 Points: 165, Level: 3
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Feb 2009
Location: Pune (India)
Posts: 11
Thanks: 0
Thanked 5 Times in 3 Posts
Default Re: Autoclave Help

Quote:
Originally Posted by gatrellm View Post
I am trying to teach high school biology in a school with a serious lack of equipment. Can I sterilize nutrient agar without one? Any suggestions

Gatrellm
If your school lack those expensive autoclave , You can use pressure cooker as the option which also work on the smae principle.

Please try to follow good laboratory practices!
Reply With Quote
  #19  
Old 06-04-2010, 01:08 PM
Pipette Filler
Points: 23, Level: 1 Points: 23, Level: 1 Points: 23, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2010
Posts: 2
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Autoclave Help

use pressure cooker,we used to use it from our college when we lack space in our autoclave,it really works
Reply With Quote
  #20  
Old 06-04-2010, 02:42 PM
Graduate Student
Points: 2,626, Level: 33 Points: 2,626, Level: 33 Points: 2,626, Level: 33
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2010
Location: Santiago, Chile
Posts: 100
Thanks: 1
Thanked 24 Times in 24 Posts
Default Re: Autoclave Help

Quote:
Originally Posted by Aga View Post
we're not talking about reference analyses but about replacing an autoclave.
I think this is what matters... take it easy, Jorge. It's just a high school class, y'know.

Pressure cooker would work kinda fine in most cases, and it'd be the best solution if you lack the autoclave. Obviously it's not the same, but physical principles are the same.

And about tyndalization... why don't you just liquid media if repeatedly heating agar is not recommended?
Reply With Quote
Reply

Tags
autoclave


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Contamination of 7H11 agar plates martin rao Microbiology Forum 4 10-31-2010 08:39 PM
Autoclave Bag johan.apryanto Lab Equipment Discussion and Reviews 0 01-23-2009 08:38 AM
Bench top autoclave ebrenner Lab Equipment Discussion and Reviews 1 01-23-2009 08:10 AM
About TE and Tris-HCl sterilize with autoclave fabio Protocols and Methods Forum 8 11-07-2004 06:29 PM
Bacterial contamination- need some help Aaron Hicks Microbiology Forum 4 08-07-2003 08:55 AM


All times are GMT. The time now is 01:55 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.17677 seconds with 16 queries