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#1
01-23-2009, 12:33 AM
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 (no subject) I*am looking to identify specific bacterial metabolites that cause malodor and then measure those byproducts by GC or other analytical method.* But I am finding it difficult to find those specific by-products.* Most interested in by-products of Staph and E. coli.* We have looked at ammonia and Proteus, measuring with Drager columns, but I want something more sophisticated.* Any ideas?  |
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#2
01-23-2009, 08:51 PM
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| (no subject) On Thu, 22 Jan 2009 17:33:00 -0800 (PST), Kate Hawley <[Only registered users see links. ]> wrote: Take a culture that you find malodorous. Run the headspace gas thru GC-MS. bob |
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#3
01-25-2009, 11:41 AM
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| (no subject) On Jan 24, 5:51*am, Bob <[Only registered users see links. ].com> wrote: Remove the contaminating dominant products first through depletion via negative column-exchange chromatography. Then do GC-MS as Bob suggested. sz |
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#4
01-26-2009, 01:00 PM
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| Re: (no subject) Do some homework 1st. You're nt the 1st to have considered this.. For the E. coli - think skatole, indole, H2S. For staph consider volatile fatty acids. |
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