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Microbiology Forum Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum.


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(no subject) - Microbiology Forum

(no subject) - Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum.


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  #1  
Old 01-23-2009, 01:33 AM
Kate Hawley
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I*am looking to identify specific bacterial metabolites that cause malodor and then measure those byproducts by GC or other analytical method.* But I am finding it difficult to find those specific by-products.* Most interested in by-products of Staph and E. coli.* We have looked at ammonia and Proteus, measuring with Drager columns, but I want something more sophisticated.* Any ideas?




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Old 01-23-2009, 09:51 PM
Bob
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On Thu, 22 Jan 2009 17:33:00 -0800 (PST), Kate Hawley
<[Only registered users see links. ]> wrote:



Take a culture that you find malodorous. Run the headspace gas thru
GC-MS.

bob





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Old 01-25-2009, 12:41 PM
shiraz14
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On Jan 24, 5:51*am, Bob <[Only registered users see links. ].com> wrote:

Remove the contaminating dominant products first through depletion via
negative column-exchange chromatography. Then do GC-MS as Bob
suggested.

sz
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Old 01-26-2009, 02:00 PM
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Do some homework 1st. You're nt the 1st to have considered this.. For the E. coli - think skatole, indole, H2S. For staph consider volatile fatty acids.
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