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Kate Hawley 01-23-2009 01:33 AM

(no subject)
 








I*am looking to identify specific bacterial metabolites that cause malodor and then measure those byproducts by GC or other analytical method.* But I am finding it difficult to find those specific by-products.* Most interested in by-products of Staph and E. coli.* We have looked at ammonia and Proteus, measuring with Drager columns, but I want something more sophisticated.* Any ideas?





Bob 01-23-2009 09:51 PM

(no subject)
 
On Thu, 22 Jan 2009 17:33:00 -0800 (PST), Kate Hawley
<[Only registered and activated users can see links. Click Here To Register...]> wrote:



Take a culture that you find malodorous. Run the headspace gas thru
GC-MS.

bob






shiraz14 01-25-2009 12:41 PM

(no subject)
 
On Jan 24, 5:51*am, Bob <[Only registered and activated users can see links. Click Here To Register...].com> wrote:

Remove the contaminating dominant products first through depletion via
negative column-exchange chromatography. Then do GC-MS as Bob
suggested.

sz

Jorge1907 01-26-2009 02:00 PM

Re: (no subject)
 
Do some homework 1st. You're nt the 1st to have considered this.. For the E. coli - think skatole, indole, H2S. For staph consider volatile fatty acids.


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