Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Animal and Molecular Model Systems > Microbiology Forum
Register Search Today's Posts Mark Forums Read

Microbiology Forum Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum.


Question For A Medical Lab Tech...

Question For A Medical Lab Tech... - Microbiology Forum

Question For A Medical Lab Tech... - Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 01-20-2009, 09:00 PM
Pipette Filler
Points: 1,886, Level: 26 Points: 1,886, Level: 26 Points: 1,886, Level: 26
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2009
Posts: 3
Thanks: 0
Thanked 0 Times in 0 Posts
Default Question For A Medical Lab Tech...



Hello everyone,

I have a very important question for a medical lab technician concerning gram stain. I is my understanding that when a gram stain test is done on a penile discharge swab, the test is "looking" for bacteria. In fact, this test is designed to detect the presence of bacteria, and also to determine if they are gram positive or gram negative.

My question is would this test detect the presence of any sperm?

The reason I am asking is because I believe I've been leaking seminal fluid for a long time. My lab report shows 3+ gram positive cocci in clusters, 4+ gram positive cocci chains, 1+ gram positive diphtheroid like bacilli, 1+ white blood cells, and 4+ epithelial cells.
Under "organisms isolated", Enterococcus faecalis is listed.
Even with this bacteria present, I believe I was leaking seminal fluid. Is this possible? Thanks for any help with this matter.
Reply With Quote
  #2  
Old 01-24-2009, 01:50 AM
jtendres's Avatar
Pipette Filler
Points: 650, Level: 12 Points: 650, Level: 12 Points: 650, Level: 12
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Apr 2008
Location: Folsom, CA
Posts: 15
Thanks: 0
Thanked 2 Times in 2 Posts
Default Re: Question For A Medical Lab Tech...

No, a sperm cell would not come out gram negative or positive because the gram stain binds to peptidoglycan in the bacterial cell wall and eukaryotic cells do not have this material and no cell wall.
Reply With Quote
The Following User Says Thank You to jtendres For This Useful Post:
admin (01-25-2009)
  #3  
Old 01-24-2009, 10:04 PM
Pipette Filler
Points: 1,886, Level: 26 Points: 1,886, Level: 26 Points: 1,886, Level: 26
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2009
Posts: 3
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Question For A Medical Lab Tech...

Thanks for your reply.

This confirms what I've been researching. Since sperm cells have cell membranes, and not cell walls, they do not contain any peptidoglycan, and thus would not stain. Also, the grams stain test looks specifically for bacteria.

They automatically assume that if a patient has discharge, it must be caused by a bacterial infection.
Reply With Quote
  #4  
Old 01-25-2009, 07:22 AM
NATHANALANPATRICKROGERS's Avatar
Summer Student
Points: 306, Level: 6 Points: 306, Level: 6 Points: 306, Level: 6
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2009
Location: Maroochydore
Posts: 73
Thanks: 4
Thanked 13 Times in 11 Posts
Default Re: Question For A Medical Lab Tech...

Quote:
Originally Posted by pinballdoctor View Post
Hello everyone,

I is my understanding that when a gram stain test is done on a penile discharge swab, the test is "looking" for bacteria. In fact, this test is designed to detect the presence of bacteria, and also to determine if they are gram positive or gram negative.

The reason I am asking is because I believe I've been leaking seminal fluid for a long time.
The contents of your swab will be cultured on agar plates to test for the presence of common and viable bacteria. Based on this evidence, a series of biochemical e.g gram and/or genomic tests follow to determine the species present. Rarer species are less likely to be detected without specific direction from your doctor.

Seminal fluid leakage is common in young men/women that are predominantly sexually inactive. However, it also tends to be an early-indicator of sexually-transmitted infections so take care.
Reply With Quote
  #5  
Old 01-26-2009, 12:33 AM
Ph.D Doctorate
Points: 3,253, Level: 37 Points: 3,253, Level: 37 Points: 3,253, Level: 37
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jun 2008
Posts: 154
Thanks: 0
Thanked 17 Times in 16 Posts
Default Re: Question For A Medical Lab Tech...

Wrong - jt. Gram's method includes 2 stains and sperm as well as fungi are stained.
Reply With Quote
  #6  
Old 08-03-2011, 09:02 PM
Pipette Filler
Points: 113, Level: 2 Points: 113, Level: 2 Points: 113, Level: 2
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Aug 2011
Posts: 6
Thanks: 0
Thanked 2 Times in 2 Posts
Default Re: Question For A Medical Lab Tech...

It sounds like you had a penile drainage culture performed.
Everything shows up on a gram stain so yes you would see
sperm. They may not report sperm when seen however as it is typical and
really has nothing to do with your infection as far as the micro. lab is concerned.
The strep that was isolated is probably from feces. You may
want to think back as to when this may have been introduced into your
penis and try to stayaway from this activity, even if it is swimming in
a pond.
Ampicillin, Amoxicillin, sulfa drugs and others are a pretty good bet as
the antibiotic of choice to help you but if you have "leaked" for months on end
it could be something more serious.
Take your antibiotics (all of them) exactly as prescribed. If your pipe
isn't fixed by then let your doctor know and he will run further tests
for std's.
Reply With Quote
  #7  
Old 08-04-2011, 06:26 PM
Pipette Filler
Points: 1,886, Level: 26 Points: 1,886, Level: 26 Points: 1,886, Level: 26
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2009
Posts: 3
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Question For A Medical Lab Tech...

UPDATE

Lab report states:

Organisms Isolated

1. Enterococcus faecalis ***** Heavy Growth


Antibiotics:

Ampicillin
Vancomycin

Comments:

With light growth of skin flora.


Source: Cult, Surf Wd, Penile discharge swab

Gram Stain: 3+ Gram Positive Cocci in clusters

4+ Gram Positive Cocci chains

1+ Gram Positive diphtheroid like bacilli

1+ White Blood Cells

4+ Epithelial Cells


* Not sure what all the above means, but am no longer leaking. The only thing I am concerned with is besides the leak, there were absolutely no symptoms... no pain, no burning, nothing.
Reply With Quote
  #8  
Old 09-09-2011, 07:10 PM
Pipette Filler
Points: 113, Level: 2 Points: 113, Level: 2 Points: 113, Level: 2
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Aug 2011
Posts: 6
Thanks: 0
Thanked 2 Times in 2 Posts
Default Re: Question For A Medical Lab Tech...

neither men nor woman can have symptoms of a UTI when in fact one is raging. I see it everyday. If there are symptoms, both sexes have burning on urination and women may have flank pain.
This is not to say you had a UTI. You could have had a seminal infection or other source infection.
I doubt they gave you vancomycin as it is expensive and given IV over long periods of time.
The ampicillin will and did work. Keep your johnson clean, especially if you have foreskin and wash it well after sex.
Reply With Quote
Reply

Tags
lab , medical , question , tech


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
~~~ ASSOCIATION ~~~ lindallalbincochrane@gmail.com Forum Physik 1 02-18-2013 07:21 AM
7th Georgia Tech - ORNL Conference on Bioinformatics Georgia Tech Conference Announcement Protein Forum 0 08-26-2009 07:13 PM
Medical Schools Colleges in India - Admissions chetan567 Medical School 0 04-10-2009 04:03 PM
Do not make Medical Writing your headache............. Priti Microbiology Forum 0 04-12-2008 01:07 PM


All times are GMT. The time now is 03:25 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.17177 seconds with 16 queries