| | Re: Microbiology Pour Plate
1) Make up your media to whatever recipe your lab uses. Add agar and autoclave.
2) Remove hot media from the autoclave and let cool until you can touch the side of the flask/beaker for 5 seconds, add appropriate filter-sterilized heat-sensitive components, such as antibiotic or supplemental carbon source.
3) Ethanol wipe down work surface, set up bunsen burner for updraft.
4) Slowly pour media into sterile plates to desired depth (1/4 inch for our bacterial plates, 1/2 inch for our yeast plates). If there are many bubbles on the surface, pick up the burner and quickly flame the surface of the media to pop bubbles. Have the lid off the plates for as short a time as possible.
5) Let cool on the bench overnight to allow some of the extra moisture to escape. Wrap in plastic (we use the sleeves/bags the plates are shipped in), label and store at 4 degrees.