Microbiology Pour Plate

[orestis]

Hiya dudes
can any1 tell me how to PROPERLY pour plates for microbiology culture plz?

[kmunson779]

1) Make up your media to whatever recipe your lab uses. Add agar and autoclave.
2) Remove hot media from the autoclave and let cool until you can touch the side of the flask/beaker for 5 seconds, add appropriate filter-sterilized heat-sensitive components, such as antibiotic or supplemental carbon source.
3) Ethanol wipe down work surface, set up bunsen burner for updraft.
4) Slowly pour media into sterile plates to desired depth (1/4 inch for our bacterial plates, 1/2 inch for our yeast plates). If there are many bubbles on the surface, pick up the burner and quickly flame the surface of the media to pop bubbles. Have the lid off the plates for as short a time as possible.
5) Let cool on the bench overnight to allow some of the extra moisture to escape. Wrap in plastic (we use the sleeves/bags the plates are shipped in), label and store at 4 degrees.

[marcus^^]

1. prepared your media, working place and apparatus to be used.
2. pipette in your culture/broth in to your petri dish.
3. pour a pre-cooled media (around 40-45 degree Celsius) in to the plate for 20ml
4. then swirl the plate in "8" shape (in a gentle manner to avoid bubbles and spill out) to homogenize your sample and the media
5. let it solidified and put into incubator`~

hope this do helps u~~