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bacteria e. coli/pseudomonas? blue staining of plates

bacteria e. coli/pseudomonas? blue staining of plates - Microbiology Forum

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Old 11-07-2008, 03:11 PM
Pipette Filler
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Unhappy bacteria e. coli/pseudomonas? blue staining of plates



Hi,
I'm growing some E.coli bacteria on LB medium with ampicillin and I recently observed a blue/green staining of the plates after one night of growth, which starts where the bacteria have grown, and then diffuses to the all plate. I was told it might be some Pseudomonas instead of E coli, but I'm a bit confused as I observed that for 12 different strains (independant, but grown at the same time), and the colonies look very similar to E coli colonies. So I think a contamination of my strains by Pseudomonas is unlikely but who knows..
Has anybody observed anything like that with E coli? Do you think they are actually Pseudomonas? How can I distinguish easily some E coli colonies from Pseudomonas?
I hope somebody could help me as it is very confusing for me....
Thanks a lot!
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Old 11-13-2008, 07:20 AM
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Default Re: bacteria e. coli/pseudomonas? blue staining of plates

You could take a few colonies and do an oxidase test since psuedomonas is an aerobe and will be oxidase positive and E.coli is a faculatative anaerobe and will be oxidase negative. Someone should have an oxidase strip box somewhere. The positive result = a dark blue color within 10 seconds.
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