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02-04-2008, 02:58 PM
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 Re: BL21 vs BL21 codon plus BL21 are fine for many proteins. If you're trying to express a human codon-optimized protein, and are not getting good yields with normal BL21s, the codon plus strain may help, as it carries tRNA genes for human codons that the bacteria have at very low levels. I suggest reading Stratagene's manual (page 7) for a discussion of the exact genes included and the different types of CodonPlus cells.        |
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02-04-2008, 05:38 PM
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| Re: BL21 vs BL21 codon plus Hi thanks for your reply. these are the basic type of information and a life scientist must know all these things so that he could work better at benchtop. regards aftab |
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