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BL21 vs BL21 codon plus

BL21 vs BL21 codon plus - Microbiology Forum

BL21 vs BL21 codon plus - Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum.


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Old 02-03-2008, 10:55 AM
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Default BL21 vs BL21 codon plus



Hi

what are the differences and advantages of using BL21 and BL21 codon plus E.coli strains for expression.

regards
aftab
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Old 02-04-2008, 03:58 PM
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Default Re: BL21 vs BL21 codon plus

BL21 are fine for many proteins. If you're trying to express a human codon-optimized protein, and are not getting good yields with normal BL21s, the codon plus strain may help, as it carries tRNA genes for human codons that the bacteria have at very low levels.

I suggest reading [Only registered users see links. ] (page 7) for a discussion of the exact genes included and the different types of CodonPlus cells.
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Old 02-04-2008, 06:38 PM
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Default Re: BL21 vs BL21 codon plus

Hi
thanks for your reply. these are the basic type of information and a life scientist must know all these things so that he could work better at benchtop.

regards
aftab
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