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Synthetic media for E.coli

Synthetic media for E.coli - Microbiology Forum

Synthetic media for E.coli - Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum.


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Old 11-19-2007, 05:52 PM
Pipette Filler
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Default Synthetic media for E.coli



Hi all, I am looking for a synthetic media recipe to grow E.coli.
I would like to monitor the effect of phosphorus on growth thus phosphorus would be kind of a limiting factor. Anyone has a idea where I should look.

Thanks,

apari006
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Old 11-20-2007, 06:55 AM
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Default Re: Synthetic media for E.coli

Hi
for the growth of E.coli we normally use L.B. Media and Nutrient media can also be used. but if you selectively want to check the effect of phosphorous on the growth of E.coli then you have to use a mininal media with different concentration of phosphorous.
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aftab
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Old 11-20-2007, 04:43 PM
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Default Re: Synthetic media for E.coli

Thanks aftabac,

That is what I was planning to do but I wanted to know if there was any recipe that would be better than another. I will probably go for ammonium sulfate, glucose, potassium hydrogen phosphate and magnesium sulfate. Variying the quantity of K2HPO4 should yield the expected results wrt phosphorus concentration.

apari006
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