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Pouring Agar Plates

Pouring Agar Plates - Microbiology Forum

Pouring Agar Plates - Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum.


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  #1  
Old 11-09-2007, 04:03 PM
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Question Pouring Agar Plates



Good day everyone,
I have some frustrating occurances about the lab. Every time I try to pour Agar plates either they have gaps (no agar), generate water in the fridge, or are too thick!

Does anyone (microbiology experts) know how to pour agar plates well?

thanks guys
clokke
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Old 11-09-2007, 04:46 PM
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Default Re: Pouring Agar Plates

Once you've autoclaved your media, put the bottles in ~37C water bath, before you pour place on stir plate for ~1min, then pour into the plates in sterile hood. You should pour one at a time, enough to cover the bottom of the plate. Leave in hood, (should be a level surface) until they solidify.
Enough experience will eventually lead you to making them perfectly.
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Old 11-09-2007, 05:25 PM
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Default Re: Pouring Agar Plates

Another quick tip: if you're getting a lot of condensation on the plates, store them upside down (agar side on top) in the fridge, then before use, quickly shake off the water pooling on the lid (into the trash can or onto the floor).

Obviously, you need to make sure they've solidified before you put them upside down.
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Old 11-13-2007, 03:41 PM
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Default Re: Pouring Agar Plates

well
good suggestion. well i would like to add that after autoclaving your media, touch it after some time if it is bearable on hand then pour it in plate in a way that i should cover the bottom, this should be done in hood and place the lid along, dont cover it immedatly. when the agarose get solidify then put the lid over and seal it with parafilm and then put in fridge upside down, i.e agar on top and lid on downside.
i am sure it will help you.
regards
aftab
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Old 11-20-2007, 04:55 PM
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Default Re: Pouring Agar Plates

Autoclave the agar and then aseptically transfer the liquid agar into seperate 1000mL flasks. Place under the antibiotic hood until cool enough to touch for more around 5 seconds. Pour enough to cover the bottom (about 1/8 ") and then IMMEDIATELY place the lid on the plate. Bacteria are everywhere and if you leave the lid off even after it cools bacteria will settle on the solid agar and contaminate the plate. After the agar solidifies place it upside down in the fridge to keep water from collecting in the agar.
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Old 01-13-2008, 01:46 PM
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Default Re: Pouring Agar Plates

To make them not to thick I'm pouring agar in until about 2/3 of the dish is covered. The agar will extend to the empty area.
If its in your budget I suggest an automatic system (e.g. AAS great device!) or you may buy ready-for-use agar dishes.
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Old 09-28-2008, 03:11 PM
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Default Re: Pouring Agar Plates

Quote:
Originally Posted by clokke76 View Post
Good day everyone,
I have some frustrating occurrences about the lab. Every time I try to pour Agar plates either they have gaps (no agar), generate water in the fridge, or are too thick!

Does anyone (microbiology experts) know how to pour agar plates well?

thanks guys
clokke
1. agar gaps: swirl it for a moment and make sure the agar covered the plate surface
2. generate water in fridge: obviously, your pouring temperature for agar is too high, make it around 40 degree C would be nice~~
3. it need experience and what kind of thickness that you wishing for...
for me i pour until agar just nice to cover all the plates~~
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Old 10-02-2008, 05:20 AM
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Default Re: Pouring Agar Plates

Make sure you are cooling the agar to around 50 degrees celsius otherwise it might start to solidify. And if need to add antibiotics after autoclaving, then add a stir bar into the agar mix before autoclaving so when you add the antibiotics you can put the flask, or what have you, onto a heating block with a stirring mechanism, so this creates less bubbling when mixing the antibiotics in. Otherwise you can use a bunsen burner to remove bubbles on the agar after you have poured it into the petri dish. When pouring in the agar, you only need to fill the entire bottom of the plate, so stop pouring when the bottom is covered. This will prevent it from being too thick.
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Old 10-31-2010, 08:39 PM
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Default Re: Pouring Agar Plates

im proud of u alll
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