Question on a MacFarland Standards

Hi, Mr. Friedel

I saw you e-mail on the internet when I was searching for how to create
microorganism supension with a know population? I know that some people
use MacFarland Standard, but We don't have MacFarland Standard, we have
Densichek colorimeter by biomeriuex. Please, would you tell me how can
I prepare those kinds of microbial suspension given a know concentration
(for example, 10e6, 10e7 or 10e8).

Thank you very much,

Luis Rivera Morales
Associate Microbiologist/Sterilization
MPROC-Juncos
Phone: 787-561-2840
Fax: 787-561-2413



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On 2008-10-10 17:08:25 -0400, "Rivera Morales, Luis [JR]"
<[Only registered users see links. ]> said:


Luis,
The bioMeriuex colorimeter usually comes with 4 tubes with McFarland
Standards 0.5, 1, 2, 3 and 4. These are made with latex particles and
are very stable.The colorimeter should have colored sections on it
relating to the 4 standards.

You can also make your own standards using a formula with Barium
Chloride and H2S04. A Google search found several sites that had the
recipie, and Wikepedia even has a chart of each standard up to 4 with
approximate bacterial concentratioin and nephelometer transmittance and
absorbance for each one. I tried to copy it into this message but it
did not format correctly.


--.
John Gentile
Newsletter editor
Rhode Island Apple Group
--
John Gentile MS, M(ASCP)
Laboratory Information Mgr.
VA Medical Center
Providence, RI
[Only registered users see links. ]

I went to Vitek2 training at bioMerieux in 2007. We were told in our
training that the Densicheck is not a true McFarland standard, so you
cannot substitute it for a McFarland.

I believe we buy our McFarland's commercially. They have about a year
outdate.

Judy Dilworth, M.T. (ASCP)
Microbiology

"Rivera Morales, Luis [JR]" <[Only registered users see links. ]> wrote in message
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