Hygromycin marker for Non tuberculous mycobacteria

I am writting to ask about what concentrations you have found to be
effective when selecting for transformants of Non tuberculous
mycobacteria such as M.avium,abscessus and chelonae.

Currently we have been using 50 and 100 ucl/ml but we keep getting
colonies growing effectively rendering selection a complete waste of
time.Apparently selection should work at 50ucl/ml.Do you have any
suggestions for any other antibiotic apart from kanamycin?

Thank you

Grace.

On Thu, 14 Aug 2008 13:39:00 +0100, "Mvenge, Nyasha"
<[Only registered users see links. ].uk> wrote:


It can vary -- for various reasons. Test it, and see what works for
you under your conditions.

Are you getting 100% colonies at the levels you are using? Or some
reduced number? If growth is still good even at fairly high levels, it
is possible that you happen to have a resistant strain.

smegmatis is one of the more common non-tb strains. There is probably
a lot of literature with it.

bob