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Problem with cultivation on anaerobic solid media

Problem with cultivation on anaerobic solid media - Microbiology Forum

Problem with cultivation on anaerobic solid media - Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum.


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Old 11-14-2007, 08:04 PM
Luzan Tatiana
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Default Problem with cultivation on anaerobic solid media



In my lab we use hungate bottles only for the liquid anaerobic media and bi-
layer solid media. We have to do inoculations on solid meida only using
Petri dishes in the anaerobic jar supplemented with gas-packs or controlled
atmosphere anaerobic chamber. THere is a problem in the chamber: the cag
mixture we use is 10% Hydrogen, 15% CO2 and Nitrogen as the rest. While
incubating plates inside, the media dries up very quickly and an enourmous
condensation is forming! IT is almost a lake of water there.

IS there any product which would absorb an acess condensate?
Thanks a lot

--

Tanya Luzan
Research Assistant
Department of Biology
University of Louisiana at Lafayette
Phone (Office): 482-5056

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Old 11-14-2007, 11:29 PM
N10
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Default Problem with cultivation on anaerobic solid media


"Luzan Tatiana" <txl8326@louisiana.edu> wrote in message
news:mailman.615.1195074105.23109.microbio@net.bio .net...


Hi Luzan

How long are you incubating for ? I use a similar system and have no
problems for up to 48 hours at 37'c

You could try a Petri dish containg a layer of Silica gel ( dried) to
absorb the humidity.

Obvioulsy check for zero effects on colony yield

Best Des


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