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Water Crystals in Frozen Bacteria!

Water Crystals in Frozen Bacteria! - Microbiology Forum

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Old 10-03-2007, 08:33 PM
tarun gupta
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Default Water Crystals in Frozen Bacteria!



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Dear Friends,

I have a small query of scientific nature.

I remember once my mom made milk based ice cream for me and kept it at 4degrees in refrigerator. The next thing I found was that ice cream was notsmooth and there were water crystals formed.

Now, my query has two parts:

1. Biological Cells have a large volume of water as its consituents. I have been wondering that when we store our bacterial plates in frozen condition at -80 degrees, don't this water forms sharp crystals and damage the cells?

If crystals do form, they should damage cellular membranes and macromolecules like proteins and DNA (suspended in solvent, ain't they?)! Logically, this should happen, shouldn't it?

2. If this does happen, how do these frozen bacteria revive when we bring them back to normaltemperatures in appropriate media? Any comments ?

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Kind Regards,
Tarun Gupta

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MSc Human Genomics
National Centre of Human Genome Studies and Research
Panjab University
Chandigarh-India

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