Dr. Michael Strohdeicher
We are two biochemistry students from Oporto, Portugal. We are developing a
microbiology project that involves the study of the complex NADH:ubiquinona
oxidoreductase from Zymomonas mobilis.In this theoretical project we´re
trying to eliminate the expression of this enzyme by doing random
mutagenesis. As you certanly know, we need some method to do a selection of
the wanted mutant. We think we can use the method you mention on the
abstract of the paper "Electron transport chain of Zymomonas mobilis"
(1990), for the removal and reincorporation of ubiquinone from the bacteria
membrane. If it wouldn´t be much of a burden,it will be a great help if you
send us the paper.
Thank you very much for your attention.
António José Ribeiro
Sandro Dá Mesquita