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| Hi coleagues, I would be much grateful if someone share some info on how a simple resin such as Chelex 100 is able to aid in the lysis of Gram+ bacteria and endospores. For example many authors use chelex-based methods to extract DNA from Bacillus spores. In the article by A Stein and D Raoult. A simple method for amplification of DNA from paraffin-embedded tissues. Nucleic Acids Res. 1992 October 11; 20(19): 5237–5238. is noted that a simple boiling did not produce PCR amplifiable DNA where as boiling in Chelex did. I know that the resin binds a large portion of the PCR inhibitors but how is it supposed to participate in the lysis step ? The first two articles that mention chelex 100 as a reagent for DNA isolation are: de Lamballerie X, Zandotti C, Vignoli C, Bollet C, de Micco P.A one-step microbial DNA extraction method using "Chelex 100" suitable for gene amplification. Res Microbiol. 1992 Oct;143(8):785-90. Walsh, P.S., Metzger, D. A., Higuchi, R., 1991. ChelexТ 100 as a medium for simple extraction of DNA for PCR based typing from forensic material. BioTechniques, 4, 506-513. Unfortunately I have not access to them and can't find em anywhere. If anybody is able to attach please help. Ivan |
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| cell , chelex100 , dna , extraction , lysis , mechanism |
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