Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Animal and Molecular Model Systems > Microbiology Forum
Register Search Today's Posts Mark Forums Read

Microbiology Forum Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum.


a question about PCR

a question about PCR - Microbiology Forum

a question about PCR - Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 06-08-2006, 11:29 AM
Fan Cheung
Guest
 
Posts: n/a
Default a question about PCR



I am a novice in this field. Can anybody give me the answer to the
following question?
During the PCR process,the primer that is complementary to the desired
sequence is very important,right? Then if the original DNA contains
another sequence which also has complementary parts to the primer but
not our target ,how can we ensure the final ampication is what we are
looking for?
Thanks.

Reply With Quote
  #2  
Old 06-09-2006, 02:44 AM
Bob
Guest
 
Posts: n/a
Default a question about PCR

On Thu, 8 Jun 2006 19:29:15 +0800, "Fan Cheung"
<[Only registered users see links. ]> wrote:


right



Well, that is up to you to check.

In part, this depends on whether you are dealing with known sequences
or unknown sequences. With known sequences, part of primer design is
to design primers that are specific for the intended sequence. This
may include a prediction as to the expected length of amplified
product.

With unknown DNA, you have to be extra careful to analyze the product
and show it is what you intended. Sequencing it is often appropriate.

Remember, you amplify what is between two primers. So simply having
one primer that has an extraneous binding site per se does not give an
extra discrete product. And sometimes, it may even be interesting that
a primer pair gives an extra product. Perhaps there is a 2nd copy of
the gene, or of a related gene.

In any case, good question. And the bottom line answer is that you
must consider the possibility, and must ensure that your product is
what you intended.

bob

Reply With Quote
Reply

Tags
pcr , question


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
question about phenol/water saturated solution Z.L. K Protocols and Methods Forum 2 02-11-2008 01:05 AM
Question about work and friction Robert Physics Forum 2 01-04-2008 12:50 PM
Just a big question... rahemanvelji@yahoo.ca Physics Forum 8 05-09-2005 08:44 PM
Sci.chem FAQ - Part 1 of 7 Bruce Hamilton Chemistry Forum 0 01-15-2004 09:06 AM


All times are GMT. The time now is 12:35 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.12077 seconds with 16 queries