Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Animal and Molecular Model Systems > Microbiology Forum
Register Search Today's Posts Mark Forums Read

Microbiology Forum Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum.


Spectro for growth curves

Spectro for growth curves - Microbiology Forum

Spectro for growth curves - Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 07-02-2005, 05:55 AM
Scott Coutts
Guest
 
Posts: n/a
Default Spectro for growth curves



Hi Folks,

We're looking at buying some new uv/vis specs soon, and I'm looking at
the options at the moment... does anyone know if there's a spec in which
continuous growth curves can be measured and plotted, rather than taking
an aliquot and measuring it at intervals? The bugs we need to measure
need to be stirred and grown at particular temperatures, too, which
complicates things. I think they exist, but I've never used one and I
dont know who makes them.

Cheers,

Scott.
Reply With Quote
  #2  
Old 07-02-2005, 02:24 PM
Larry D. Farrell
Guest
 
Posts: n/a
Default Spectro for growth curves

Why not simply grow your cultures in nephelometer flasks and take the
absorbance in whatever spec you wish? That way, you don't have to
aliquot and discard, and the culture can be maintained at the necessary
temperature and agitation except for the few seconds needed for the
actual measurement.

Scott Coutts wrote:

Reply With Quote
  #3  
Old 07-03-2005, 07:03 AM
Scott Coutts
Guest
 
Posts: n/a
Default Spectro for growth curves


Hi Larry,

Are they the flasks with the sealed tube projecting from the neck? If
so, yeah, I'd considered them, but it would be more convenient to have
one that allows us to automate the procedure. Since we're buying new
specs anyway, I thought it would be best to see if we can get a model
that will do the widest range of things we need (: The thing is, I'm
pretty sure they exist, I'm just not sure who makes them.

Scott.

Larry D. Farrell wrote:
Reply With Quote
  #4  
Old 07-04-2005, 07:56 AM
Lesley Robertson
Guest
 
Posts: n/a
Default Spectro for growth curves


"Scott Coutts" <[Only registered users see links. ].au> wrote in message
news:V4Mxe.12898$[Only registered users see links. ].au...
There's a few things it would be useful to know - expected volumes, for a
start. How critical will dissolved oxygen be? Most of the automative reading
apparatus we had in the past could only manage a very sedate shaking rate,
which meant that oxygen was frequently limiting as the cultures matured...
Some years back, I was fortunate to use a spectrophotometer at the Free
University of Amsterdam which had been adapted to take a full-sized
continuous culture system (it was used for measuring cytochromes oxidation
levels at steady state) - 1 litre pot and all. I have an idea that they did
the adaptions themselves, but I'll try and check - that particular Prof has
now retired.
Lesley Robertson


Reply With Quote
  #5  
Old 07-04-2005, 11:39 AM
Scott Coutts
Guest
 
Posts: n/a
Default Spectro for growth curves

Lesley Robertson wrote:

Hi,

Thanks for your help. I've discovered a few systems, several from
Thermo, several from Cecil, and one or two from Beckman. Most of them
take samples from either a custom vessel that comes with the instrument
(these are often actually designed for pharmaceutical tablet dissolution
monitoring) or a user-supplied vessel. Others use a probe that can be
inserted into any vessel. The best I've seen is a stirred, temperature
controlled (peltier) cuvette that fits inside the spec.

Cheers,

Scott.

Reply With Quote
  #6  
Old 07-04-2005, 02:36 PM
Lesley Robertson
Guest
 
Posts: n/a
Default Spectro for growth curves


"Scott Coutts" <[Only registered users see links. ].au> wrote in message
news:2e9ye.14146$[Only registered users see links. ].au...
I have, in the past, used flow through cuvettes one a pump, so that the
culture sample is returned to the mail vessel after reading. Our problem was
that the dissolved oxygen in the tubes and cuvette fell off rapidly, so that
we could never be sure that the culture was as aerobic as we wanted.
Lesley Robertson
[Only registered users see links. ]
[Only registered users see links. ]


Reply With Quote
Reply

Tags
curves , growth , spectro


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
standard curves for duplex reaction rusty Real-Time PCR and Quantitative PCR Forum 0 07-21-2009 11:28 AM
No success generating standard curves... donchoi Real-Time PCR and Quantitative PCR Forum 1 11-24-2008 10:01 PM
Analysing Melting Curves with TaqMan probe Leowe Real-Time PCR and Quantitative PCR Forum 5 06-25-2008 05:30 PM
Data on growth curves? Pernille Zebrafish Forum 0 09-19-2006 10:49 AM
Science closing in on definition for multi-dimensional legendary catenary curves of egg surfaces: Don1 Physics Forum 4 04-18-2005 07:05 PM


All times are GMT. The time now is 09:07 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.14171 seconds with 16 queries