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Amplification product size

Amplification product size - Microbiology Forum

Amplification product size - Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum.


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Old 05-27-2004, 07:17 PM
nmesbah@uga.edu
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Default Amplification product size




I am trying to amplify total genomic DNA extracted from soil using archeal 21f and 1392r primers. I expect to get a product ~1400 bp long but I keep on getting small fragments 200-500bp long. Should I extend the extension time?

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