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Boost Protein Expression by Codon Optimization

Boost Protein Expression by Codon Optimization - Microbiology Forum

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  #1  
Old 01-07-2004, 12:44 AM
Sally Wang
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Default Boost Protein Expression by Codon Optimization



Dear Colleague,

Happy New Year!

As we know, codon preference among different species could be dramatically different. To enhance the expression level of a foreign protein in a particular expression system (E.coli, Yeast, Insect, or Mammalian cell), it is very important to adjust the codon frequency of the foreign protein to matchthat of the host expression system. One classic example is GFP (green fluorescent protein) which was optimized to achieve high-level of expression in mammalian cells.

GenScript has developed a proprietary algorithm for codon optimization. This algorithm can optimize sequences for protein expression using either your own codon usage table or those from publicly available codon usage database. It can converts your amino acid sequence into a DNA sequence with overall codon usage similar to a specified organism, and also optimizes the RNAsecondary structure, GC content, repetitive codons etc. Using Genscript optimized synthetic genes, many of our customers have reported dramatic increase on protein expression.

The optimized gene can be synthesized by GenScript gene synthesis technology with 100% fidelity. The cost is as low as $2.35 per base pair. Our service is very flexible, we can clone the optimized gene into our standard vectors (without extra charge), or any expression vector that you provide (for$400 extra charge).

Gene synthesis is a powerful technology and it has many other applications.Another application example is to replace PCR Cloning. Please visit our web ([Only registered users see links. ]) to learn more about thistechnology.

Besides Gene Synthesis, we also provide custom vector-based siRNA, siRNA cassette, peptide, oligo, cloning and protein expression, biochemical reagents, and labwares. Please visit our web site ([Only registered users see links. ]) to learn more about our services.


Sincerely,

Sally Wang
Account Manager
GenScript Corporation
120 Centennial Ave.
Piscataway, New Jersey 08854, USA
Tel: 1-732-885-9188, 1-732-357-3839
Fax: 1-732-210-0262
Email: [Only registered users see links. ]
Web: [Only registered users see links. ]


==============================================

P.S. If you prefer not to receive GenScript News and Offers in email, please follow this link:
[Only registered users see links. ], and we apologize

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Old 01-08-2004, 09:38 AM
Evert-Jan Blom
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Default Boost Protein Expression by Codon Optimization

Codon optimizer: a freeware tool for codon optimization.

[Only registered users see links. ]

Cheers

EJ


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