I'm looking to set up an experiment to determine whether or not A1
media (used for feacal coliform determination - MPN technique)with
different preparation dates produces significantly different numerical
results when inoculated with the same original sample/samples.
Media of varying ages (ie day 1, day 3, day 5, day 7, day 9, day 12,
day 15)will be used.
Any suggestions on the best way of performing this experiment?
It looks like you should work backwards and prepare your media starting with
the 15 day old stuff and then 3 days later the 12 day stuff, etc until you
get to the 1 day (actually 0 day for the freshest media). Then on that 15th
day inoculate all your tubes from the same sample. That way you can easily
see when each set shows growth. Make sure you have enough sample for all
those tubes and that it is really mixed well.
Personally I always dreaded the MPN technique and would rather do a filter
culture of 100 cc planted on Levine EMB. But I haven't done coliform counts
in many years - don't really miss it.
in article [Only registered users see links. ], Pete at [Only registered users see links. ] wrote on 1/5/04 2:32 AM:
John Gentile MS M(ASCP) [Only registered users see links. ]
Laboratory Information, QA Manager
VA Medical Center
The contents of this message are mine personally and do not reflect any
position of the Government or VA.