Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Animal and Molecular Model Systems > Microbiology Forum
Register Search Today's Posts Mark Forums Read

Microbiology Forum Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum.


Maintaining microaerobic conditions

Maintaining microaerobic conditions - Microbiology Forum

Maintaining microaerobic conditions - Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 11-05-2003, 03:35 PM
C Coward
Guest
 
Posts: n/a
Default Maintaining microaerobic conditions



Greetings microbiologists...We're looking into the possibility of
extending our capacity for growing Campylobacter. Currently we use a
microaerobic workstation to maintain 85% N2/5% O2/5% H2/5% CO2 at 42oC.
We're consdidering getting an air incubator and growing the bugs in
containers with CampyGen gas packs. We've got a couple of microbiology gas
jars but they're an awkward shape so was wondering whether there'd be any
problems with getting appropriate size boxes e.g. Tupperware, taping the
edges to attempt an air-tight seal, and using gas packs inside.

Also, are there any ways (indicator strips for example) to determine if
you've successfully generated a microaerobic environment?

TIA

Chris
Reply With Quote
  #2  
Old 11-05-2003, 04:33 PM
Lesley Robertson
Guest
 
Posts: n/a
Default Maintaining microaerobic conditions


"C Coward" <[Only registered users see links. ].cam.ac.uk> wrote in message
news:bob5c6$pso$[Only registered users see links. ].cam.ac.uk...


If you use comercially available gas packs intended for Campylobacter, you
have to be sure that the gas volume in your container is the same as the
containers for which the packs are designed, otherwise they either over- or
under- react. you also have to be very careful with the type of plastic you
use, if you go down that route as oxygen zaps happily through many types. We
used the anaerobic jars supplies by (I think) Beckton Dickenson, who also
made the packs.


I THINK the company also supplied strips. It's a few years since I used the
things. Oxoid also supplied anaerobic packs, but I can't remember if they
did microaerobic ones.
Lesley Robertson
[Only registered users see links. ]


Reply With Quote
  #3  
Old 11-05-2003, 05:38 PM
Dahd
Guest
 
Posts: n/a
Default Maintaining microaerobic conditions

I've been working with Campy for years. They are really easy to handle, at
least the way we do things.

There are companies that supply bottled gases of one sort or another for
industrial uses--e.g., places where you normally get nitrogen or CO2
cylinders.

It is possible to order cylinders with the campy gas mix --a bit expensive
($250/per bottle), but well worth it. A tank will last for 6 months or
more.

We place our plates in Zip Lock style plastic bags. I find that freezer
bags work best. We have used Glad bags, Hefty Bags, Zip Lock, etc. The
brand doesn't seem to make as much difference as whether or not the bag is a
freezer bag.

The bags are then "gased" with the campy gas. Usually, I fill and empty the
bag a couple of times to flush the air out.

This works wonderfully.

I typically grow my organism on Campy-Cefex plates as described by Stern et
al. These are Brucella Agar plates that have been spiked with Cefaperizone,
cycloheximide; and I like to add rifampicin.
Lysed horse blood is added to the plates too.

I don't want to post my name and phone number here, nor my real email
address--you can figure out who I am by doing a quick search-- Avian
Diseases 47:753-758--2003. I'd welcome a phone call from anyone who wants
to talk about campy.




"C Coward" <[Only registered users see links. ].cam.ac.uk> wrote in message
news:bob5c6$pso$[Only registered users see links. ].cam.ac.uk...


Reply With Quote
Reply

Tags
conditions , maintaining , microaerobic


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
His-tag protein purification under denaturing conditions Arabidop Molecular Biology Articles and Protocols 5 06-28-2009 06:05 AM
running ssDNA (30-1000nt) in denatured conditions TC Protocols and Methods Forum 1 11-19-2008 08:10 AM
Galileo (NOT Einstein) is inventor of Second postulate of Relativity AJAY SHARMA Physics Forum 0 11-05-2006 02:22 AM
alt.astronomy, alt.sci.physics, alt.sci.physics.new-theories, AJAY SHARMA Physics Forum 0 11-05-2006 02:20 AM
Maintaining conditioned water. David Lains Zebrafish Forum 0 05-12-2005 10:13 PM


All times are GMT. The time now is 02:16 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.13233 seconds with 16 queries