I am a PhD student in Cambridge looking to use a fluorescence microscope.
Does anyone have access to this (to use with a category 2 organism) with a
high speed camera (shutter speed of 1/500s) and an ion laser capable of
strobing at 60Hz?? I would be very grateful to use it for a short space of
time. Any costs could be paid (within reason!).
Alpa Kanji ([Only registered users see links. ].uk)
"Alpa Kanji" <[Only registered users see links. ].cam.ac.uk> wrote in message
news:bo8a89$7up$[Only registered users see links. ].cam.ac.uk...
Sorry, can't help with equipment, but the setup sounds interesting. When I
was working with fluorescent microphotography (using incident light
fluorescence) the main problem was the long exposure times needed (several
seconds), and the quenching of fluorescence that occurred. (This was quite a
long time ago.) The laser is presumably to combat the quenching, but even
so, 1/500 second seems a very short exposure. Would you care to let us know
what you'll be doing?