I was wondering wether someone is experienced using anaerobic chambers? I
want to change the palladium pellets, but the price of the original supplier
(Coy) seems to me extremely high. Does have anyone used an alternative? Can
palladium on aluminumoxid pellets (Pd 0.5%) also be used? I doubt that the
original pellets are pure palladium...
In <3f433b55$[Only registered users see links. ].ch> Markus Zimmer wrote:
I think the palladium pellets were coated beads of aluminum. We've gone
to the Mitsubishi anaerobic system that uses a dry chemical in a pouch
that activates when you expose it to air. Very simple - put the plates
in the container, put in an indicator, rip open a pouch, seal the
container and put it away. We buy ours from Remel.
Is this the system that uses the square boxes instead of jars? We want
to switch to this system but alas, our buying contract won't allow us to
buy from Remel. This hopefully will change in the future. We buy some
stuff from Remel, but just small volume items.
I haven't used an anaerobic chamber in three years. We currently put our
original anaerobic plates directly into BBL bags (with the blue liquid)
and heat seal. I have seen far more anaerobes growing with this system
on original plates than I ever did using a chamber. We keep the plates'
exposure to air at a minimum and isolates anaerobes all the time. The
other day our tech had 18 anaerobic ID's to set up. We put all
subcultures and plates (after opening the bags after 2 day incubations)
into jars. We only use the bags for initial setup.
I don't know how we'd use a chamber and record everything in a paperless
micro system (we use Misys/aka Sunquest Paperless Micro). We'd have to
write everything down and then put all the comments into the computer.
Working with the volume we do with those damned gloves or sleeves would
be awful. I don't miss them at all. We always had problems with glove
holes, sleeve tears, gas to check, etc. at my previous employer(s). We
also had a much lower volume at those labs, too.
I work in a high volume micro lab where we get 10-25 anaerobic specimens
a day. [Our total daily M-F intake of cultures/day is around the 300
mark at least. This is not counting blood cultures, which probably top
100 sets per day, and many days are over this. We do the work for four
hospital micro labs in our city, plus outreach.] They are mostly
legitimate anaerobic specimens, i.e. tissues and fluids. We keep all
anaerobic plates for five days before reporting out as "no growth" so we
have LOTS of plates. I can't imagine juggling this load in a chamber.
Just my .02.
Judy Dilworth, M.T. (ASCP)
"John Gentile" <[Only registered users see links. ]> wrote in message
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Yes, they can be regenerated. We used to put the pellets in an oven, at 180
oC for 1 1/2 hours, I think.
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Yes they are the square boxes. Our chamber is down for the count and I'm not
sure they will ever find the leak. However, using the boxes seems to be
working ok, we even isolated a Fuso which tells us that the plates are being
John Gentile Secretary, Rhode Island Apple Group [Only registered users see links. ] RIAG Web page: [Only registered users see links. ]
"I never make mistakes, I only have unexpected learning opportunities!"
Yeah, we do the same. As I posted to this message in the other group, we
routinely culture strict anaerobes and our catalysts are many years old.
We use them a single time to grow up our strains on plates, then
regenerate them before using again. We bake them out in a hot air oven.
But we're also a research lab and we dont do isolations, I should point
out. We just culture the bugs we work on (we have an anaerobe lab that
works on several strict anaerobes). But hey, if they're growing, then
our catalysts are working!