Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Omics and Genomics Forum > Microarrays Forum
Register Search Today's Posts Mark Forums Read

Microarrays Forum Microarray Forum. Discuss array technology and Gene Chips. DNA microarray and Protein arrayit forums.

Normalisation issue?

Normalisation issue? - Microarrays Forum

Normalisation issue? - Microarray Forum. Discuss array technology and Gene Chips. DNA microarray and Protein arrayit forums.

LinkBack Thread Tools Display Modes
Old 11-20-2012, 10:10 AM
Pipette Filler
Points: 66, Level: 1 Points: 66, Level: 1 Points: 66, Level: 1
Activity: 0% Activity: 0% Activity: 0%
Join Date: Nov 2012
Posts: 2
Thanks: 0
Thanked 0 Times in 0 Posts
Default Normalisation issue?


I'm quite new to microarray analysis and was hoping to get some thoughts on an issue I'm having.

I have results from Mouse Gene ST 1.0 chips, essentially grouped into tumour and non-tumour samples. I get a weird result when looking at my differentially expressed probes, I have 5 upregulated out of the first 500 listed.

I was wondering if this could be a normalisation issue.

When I look at the signal histogram from the chips after normalisation (attached) with affymetrix expression console I can see that the histograms don't overlap. My interpretation of this is that the samples aren't normalising. Is this correct? Is this why I'm getting my strange result? What can I do to overcome this issue?
Attached Images
File Type: jpg AP BMEL NTvT.jpg (19.4 KB, 1 views)
Reply With Quote

issue , normalisation

Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
qPCR NEWS April 2009 - focus on microRNA normalisation Editor www.Gene-Quantification.info Protocols and Methods Forum 0 04-29-2009 02:18 PM
[Neurofly] The Heisenberg Special Issue of J Neurogenetics Neurofly 2008 Drosophila Forum 0 02-20-2009 09:23 AM
Yeast Digest, Vol 45, Issue 2 Shaoyu Wang Yeast Forum 0 02-05-2009 07:09 PM
Proteins Digest, Vol 22, Issue 15 Dan Guire Protein Forum 0 03-31-2007 10:13 PM
Plant Physiology Arabidopsis Special Issue 2004 Robert Last Arabidopsis and Plant Biology 0 09-18-2003 10:24 PM

All times are GMT. The time now is 04:43 PM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2015, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11440 seconds with 17 queries