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How to interpret RES microarray expression file Hi all I am struggling with the RES file format and its interpretation of P/M/A calls inferences. My specific queries are : (1) For calculating expression data for a particular gene in that file, many genes are represented by more than one probe_set ids. So, I averaged probeset ids' values related to each gene. Is it right way to do this? (2) Do I have to exclude the values of expression corresponding to M and A notation ? Because, my presumption is that only P is significant and should be taken into account for calculating expression data. (3) These absolute values are fold changes (overexpression) or what ? And are these values compared with those of normal conditions; if this is the case, then what was the control/normal for each cell line? Was it same or different for each cell line? because in my data file I see all the values are above zero (0) only which give the impression that neither of gene was downregulated !! Is it the case. Or suggest me where I can proper information regarding RES file format and calculation of gene expression from this file format. Please suggest the needful Thanks KD |
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