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-   -   How to interpret RES microarray expression file (http://www.molecularstation.com/forum/microarrays-forum/86516-how-interpret-res-microarray-expression-file.html)

monu kumar 09-24-2012 04:05 PM

How to interpret RES microarray expression file
 
Hi all
I am struggling with the RES file format and its interpretation of P/M/A calls inferences. My specific queries are :
(1) For calculating expression data for a particular gene in that
file, many genes are represented by more than one probe_set ids. So, I
averaged probeset ids' values related to each gene. Is it right way to
do this?
(2) Do I have to exclude the values of expression corresponding to M
and A notation ? Because, my presumption is that only P is significant and should be taken into account for calculating expression data.
(3) These absolute values are fold changes (overexpression) or what ?
And are these values compared with those of normal conditions; if this
is the case, then what was the control/normal for each cell line? Was
it same or different for each cell line? because in my data file I see all the values are above zero (0) only which give the impression that neither of gene was downregulated !! Is it the case.

Or suggest me where I can proper information regarding RES file format and calculation of gene expression from this file format.

Please suggest the needful

Thanks
KD


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