| | Re: Explain the basic principles of how an oligonucleotide microarray operates, showi
To answer your question:
The probes correspond to short oligonucleotide sequences thought to be representative for a given gene. Each oligonucleotide is represented by two probes: one with the exact sequence and one with a mismatch nucleotide. Hybridization occurs at a critical temperature where a single nucleotide mismatch can prevent hybridization. White = strong hybridisation, black = no hybridisation. Based upon the hybridization ratio between PM and MM, it is determined whether a gene is ‘Absent’, ‘Marginal’ or ‘Present’.