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# What is meant by thresholding and image segmentation?

## What is meant by thresholding and image segmentation? - Microarrays Forum

### What is meant by thresholding and image segmentation? - Microarray Forum. Discuss array technology and Gene Chips. DNA microarray and Protein arrayit forums.

#1
04-13-2012, 05:40 PM
Pipette Filler

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What is meant by thresholding and image segmentation?

What is meant by thresholding and image segmentation? How can a histogram representation of an original microarray image be used to perform both these procedures with the goal of ‘automatically’ identifying microarray spots in the original image?

Thanks.

Last edited by ManGer; 04-13-2012 at 05:41 PM.
#2
04-13-2012, 06:22 PM
Pipette Filler

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Re: What is meant by thresholding and image segmentation?

Thresholding is an old, simple, and popular technique for image segmentation.
Image segmentation is to divide the image into disjoint homogenous regions or classes, where all the pixels in the same class must have some common characteristics:

GB (x,y) =
{1, if G (x,y) >T
{0, if G (x,y) ≤T

Where:
• G(x,y): The input gray image that we want to segment.
• GB: segmentation result. Forms a binary image, in which each value of GB(x,y) gives the category that the corresponding pixel belongs to.
• T: Threshold value. It is an integer within the range [0...K]
• G: Image.

The pixels of a microarray spot can be regarded as either just showing background noise or real signal. In a histogram of intensities (often log-transformed values are being used here) this is reflected in a bi-modal structure (camel-like curve). Statistical models can be used to define a threshold between the two modes and thus classify pixels as "background" or "signal".
 The Following User Says Thank You to Max546 For This Useful Post: ManGer (04-19-2012)

 Tags biomedical systems , histogram , image , image segmentation , meant , microarray , segmentation , thresholding

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