| | |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Microarrays Forum Microarray Forum. Discuss array technology and Gene Chips. DNA microarray and Protein arrayit forums. |
| | LinkBack | Thread Tools | Display Modes |
|
#1
| |||||||||||
| |||||||||||
| Why not just extract the DNA from the sample instead of the RNA ? |
|
#2
| |||||||||||
| |||||||||||
| DNA in the cell is genomic. When you extract RNA it can be in various forms. Specifically in this context, you are extracting mRNA which is directly mRNA expression, which in turn is correlated (but not perfectly) to protein expression. Microarrays are designed to quantify expression. cDNA is used because it is much more stable than RNA, and far easier to work with. |
|
#3
| |||||||||||
| |||||||||||
| DNA in the cell is genomic. When you extract RNA it can be in various forms. Specifically in this context, you are extracting mRNA which is directly mRNA expression, which in turn is correlated (but not perfectly) to protein expression. Microarrays are designed to quantify expression. cDNA is used because it is much more stable than RNA, and far easier to work with. |
| Tags |
| analysis , cdna , convert , experiments , extract , microarray , rna , _ |
| Thread Tools | |
| Display Modes | |
|
|