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Why do some experiments extract RNA and convert it to cDNA for microarray analysis?

Why do some experiments extract RNA and convert it to cDNA for microarray analysis? - Microarrays Forum

Why do some experiments extract RNA and convert it to cDNA for microarray analysis? - Microarray Forum. Discuss array technology and Gene Chips. DNA microarray and Protein arrayit forums.


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Old 09-30-2009, 08:16 AM
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Default Why do some experiments extract RNA and convert it to cDNA for microarray analysis?



Why not just extract the DNA from the sample instead of the RNA ?
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Old 09-30-2009, 08:16 AM
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DNA in the cell is genomic.

When you extract RNA it can be in various forms. Specifically in this context, you are extracting mRNA which is directly mRNA expression, which in turn is correlated (but not perfectly) to protein expression. Microarrays are designed to quantify expression.

cDNA is used because it is much more stable than RNA, and far easier to work with.
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Old 09-30-2009, 08:16 AM
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DNA in the cell is genomic.

When you extract RNA it can be in various forms. Specifically in this context, you are extracting mRNA which is directly mRNA expression, which in turn is correlated (but not perfectly) to protein expression. Microarrays are designed to quantify expression.

cDNA is used because it is much more stable than RNA, and far easier to work with.
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