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Microarry desgin question
I'm planning a microarray experiment using a genome from a bacterium that is not amenable to genetic manipulation. However I do have another strain of the same bacterium that I can make mutations in but that has not been sequenced. My question: 1) should I set up the microarray experiment using the sequenced strain and make mutations in interesting genes in the second strain. My problem with this is if I find a regulatory gene, I will not be able to use the microarray to determine the genes in the regulatory pathway 2) should I use the microarray to do comparitive genomic analysis to determine genomic differences. My problem with this is that even small difference (~10%) may result in a difference of ~200 genes. I've been having trouble finding what may be acceptable in the literature.
Re: Microarry desgin question
i suggest u that at least one of the strain must be completely sequenced. so that u can compare the genome with other strain using comparative genomics tools.ya u r correct u cant use the microarray technique. u can compare the two genome sequences using bioinformatics tool also.
|desgin , microarry , question|
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