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ChIP on chip Affymetrix

ChIP on chip Affymetrix - Microarrays Forum

ChIP on chip Affymetrix - Microarray Forum. Discuss array technology and Gene Chips. DNA microarray and Protein arrayit forums.


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Old 07-25-2008, 12:26 PM
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Default ChIP on chip Affymetrix



I am conducting ChIP on chip experiments on Affymetrix tiling microarrays. Using the Affymetrix protocol I amplified my chromatin immunoprecipitated DNA with dntps and dutps.

The problem is that i get signal after 1ng of DNA from my template after 30 cycles, but only signal after 34 cycles for my amplified DNA.

I am worred i may be amplifying non-specific product.
does anyone have a similar problem?

thanks
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