Protein Micorarray

aftabac · #1 (**permalink**) 05-12-2007, 12:14 PM

Hi
For DNA miroarray result we countercheck the results by real time PCR, so for Protein Microarray what is the key technique to counter check the results

regards
aftab

a_pranay · #2 (**permalink**) 05-21-2007, 06:06 PM

HI TO EVERYONE ,
I M A NEW MEMBER OF MOLECULAR STATION. I M A P.hd STUDENT and doing research in science.

aftabac · #3 (**permalink**) 05-25-2007, 11:55 AM

Hi
you are mostwelcome to forum and we hope you will enjoy this forum

best regards
aftab ahmad
Moderator, Molecular Station forum

moleculardude · #4 (**permalink**) 06-11-2007, 01:11 PM

Hello Aftab,

I would check western blot of the proteins that seem to be elevated and decreased. Also, would be a good idea to western blot proteins that are not changed as internal control (check also alpha-tubulin and actin for example)

Pulse radiolabeling and IP would be most quantitative and accurate although western blotting is more common control measure for protein microarray controls.

Very good point Aftab,
thanks!

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Protein Micorarray

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Microarray Forum. Discuss array technology and Gene Chips. DNA microarray and Protein arrayit forums.