Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Proteomics Forum > Mass Spectrometry Forum
Register Search Today's Posts Mark Forums Read

Mass Spectrometry Forum Discuss and post questions about LC-MS, Q-STAR, MALDI-TOF, and other Mass Spectrometry Questions in the Mass Spec Forum.


Inconsistency in Area of Chromatogram

Inconsistency in Area of Chromatogram - Mass Spectrometry Forum

Inconsistency in Area of Chromatogram - Discuss and post questions about LC-MS, Q-STAR, MALDI-TOF, and other Mass Spectrometry Questions in the Mass Spec Forum.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 03-15-2012, 05:51 AM
Pipette Filler
Points: 45, Level: 1 Points: 45, Level: 1 Points: 45, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Mar 2012
Posts: 9
Thanks: 0
Thanked 0 Times in 0 Posts
Default Inconsistency in Area of Chromatogram



Hi,

I am having problem in obtaining the consistent area of peaks (Chromatogram). % of CV calculated with 10 repeated readings (injected 10 times from the same vial). The % of CV is 14%. Any suggestion to trouble shoot the problem?

Thank You.
Reply With Quote
  #2  
Old 03-12-2013, 04:36 PM
Pipette Filler
Points: 67, Level: 1 Points: 67, Level: 1 Points: 67, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Mar 2013
Posts: 2
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Inconsistency in Area of Chromatogram

I'm having the same problem, only that the CV% I obtain is around 35%, the area goes from 1 200 000 to 400 000 cps in the 10th injection.

How did you solve the problem?
Reply With Quote
  #3  
Old 05-14-2013, 07:37 PM
pigfarmer's Avatar
Summer Student
Points: 3,196, Level: 36 Points: 3,196, Level: 36 Points: 3,196, Level: 36
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jun 2009
Location: Indiana
Posts: 99
Thanks: 26
Thanked 14 Times in 14 Posts
Default Re: Inconsistency in Area of Chromatogram

I have no imput for you guys on this, but if you get a solution, I'd like to hear it. Weird problem. Maybe try this forum: [Only registered users see links. ]

Hardcore people there, not just a buch of people looking for homework help
Reply With Quote
Reply

Tags
area , chromatogram , inconsistency


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Inconsistency in Area under the curve Selina Mass Spectrometry Forum 1 03-21-2012 07:40 AM
Politics And Cannibalism? Introducing The Dourties, Chelsea, Bill, Hillary, Barrack Obama, George Bush, Jr., And All Of Capital Hill! jon_johnfrancisayres@yahoo.com Microbiology Forum 0 10-06-2007 05:59 AM
GNU units and units.dat; Units of Measurement and Unit Conversion James Redford Physics Forum 0 07-31-2005 12:08 PM
Protein surface interpretation - help Stefek Borkowski Protein Forum 7 10-07-2004 01:23 PM


All times are GMT. The time now is 08:26 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.16477 seconds with 16 queries