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Developing a dopamine detection method for LC-MS/MS on a QSTAR

Developing a dopamine detection method for LC-MS/MS on a QSTAR - Mass Spectrometry Forum

Developing a dopamine detection method for LC-MS/MS on a QSTAR - Discuss and post questions about LC-MS, Q-STAR, MALDI-TOF, and other Mass Spectrometry Questions in the Mass Spec Forum.


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Old 03-14-2012, 03:50 AM
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Cool Developing a dopamine detection method for LC-MS/MS on a QSTAR



Hey,

Has anyone performed any dopamine detection work before?

I'm developing a method for detecting dopamine using an LC-MS/MS QSTAR setup.

I have been employing a HILIC column for chromatography, 10mM Ammonium acetate or formate in buffer A, 0.1% formic acid in acetonitrile in buffer B, a gradient from 90%B to 20%B, scans targeting a parent ion of m/z 154 and diagnostic fragment of m/z 91.

The results have been... varied.

Any suggestions, comments, jokes to brighten my day would be greatly appreciated.

Thanking you in advance,

Brendan.
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detection , developing , dopamine , lc-ms/ms , lcms or ms , mass spec , method , method development , qstar


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