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-   -   Developing a dopamine detection method for LC-MS/MS on a QSTAR (http://www.molecularstation.com/forum/mass-spectrometry-forum/84908-developing-dopamine-detection-method-lc-ms-ms-qstar.html)

bee-dog-swan 03-14-2012 03:50 AM

Developing a dopamine detection method for LC-MS/MS on a QSTAR
 
Hey,

Has anyone performed any dopamine detection work before?

I'm developing a method for detecting dopamine using an LC-MS/MS QSTAR setup.

I have been employing a HILIC column for chromatography, 10mM Ammonium acetate or formate in buffer A, 0.1% formic acid in acetonitrile in buffer B, a gradient from 90%B to 20%B, scans targeting a parent ion of m/z 154 and diagnostic fragment of m/z 91.

The results have been... varied.

Any suggestions, comments, jokes to brighten my day would be greatly appreciated.

Thanking you in advance,

Brendan.


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