Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (http://www.molecularstation.com/forum/)
-   Mass Spectrometry Forum (http://www.molecularstation.com/forum/mass-spectrometry-forum/)
-   -   ProteoMiner depletion of serum and Mass Spec analysis (http://www.molecularstation.com/forum/mass-spectrometry-forum/84596-proteominer-depletion-serum-mass-spec-analysis.html)

KirstenM 02-06-2012 04:10 PM

ProteoMiner depletion of serum and Mass Spec analysis
 
Hi there

I am about to deplete my bovine serum samples to remove the highly abundant proteins so I can detect low abundant proteins using Mass Spec. However the buffers used in proteominer can affect trypson digest step, has anyone ever tried anything like this? And if so, what clean up step did you apply to remove buffers that could effect Mass spec?

Thanks

Kirsten

bee-dog-swan 03-14-2012 03:37 AM

Re: ProteoMiner depletion of serum and Mass Spec analysis
 
Hey, we have been doing something similar, depleting abundant proteins from human plasma samples using the Sigma ProteoPrep Immunoaffinity Depletion kit.

The depleted samples underwent tryptic digestion without the need for any extra clean up steps, just a slight dilution in 50mM ammoniumbicarbonate.

Gustaveimj 02-12-2013 02:40 PM

Ugg 格安
 
[

[

[

Gustaveimj 02-12-2013 02:59 PM

Ugg 格安
 
[

[

[


All times are GMT. The time now is 07:29 AM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.09116 seconds with 11 queries