Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Proteomics Forum > Mass Spectrometry Forum
Register Search Today's Posts Mark Forums Read

Mass Spectrometry Forum Discuss and post questions about LC-MS, Q-STAR, MALDI-TOF, and other Mass Spectrometry Questions in the Mass Spec Forum.


poor protein score

poor protein score - Mass Spectrometry Forum

poor protein score - Discuss and post questions about LC-MS, Q-STAR, MALDI-TOF, and other Mass Spectrometry Questions in the Mass Spec Forum.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 10-17-2011, 08:12 AM
Pipette Filler
Points: 3,465, Level: 38 Points: 3,465, Level: 38 Points: 3,465, Level: 38
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Aug 2007
Location: izmir,Turkey
Posts: 27
Thanks: 0
Thanked 0 Times in 0 Posts
Default poor protein score



I have 2D spots which were stained with mass compatible silver staining method. I got reasonable MS peaks following in gel digestion, but unfortunately protein scores are lower than significancy level predicted by Mascot tool. How can I manage to increase protein score? And also, all peaks represent the same spot do not give the same result. Do the all peaks have to give same result in Mascot search? (For instance, DnaJ is the first score in a one peak, but 7th in other). It would be very much appreciated if you could help me.
Reply With Quote
Reply

Tags
poor , protein , score


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Mass Spectrometry Score and Ranking spongebob Mass Spectrometry Forum 1 10-28-2011 09:46 AM
will i get 75 to 80 percent scholarship for a GRE score of 1400 to 1500??? dark knights Molecular Biology Techniques 0 08-04-2009 05:45 AM
Earth Science Regents 2009 score? Please Help!? Bronx,10462 Environmental Sciences and Issues 0 06-24-2009 10:17 AM
score lets Maify in back of steel ralf.konzen@SYMPATHY.COM.AR Physics Forum 0 08-19-2007 09:09 AM
Similarity Score for Chemical Structures / Graphs porschekoenig@yahoo.de Organic Chemistry Forum 0 08-10-2005 02:56 PM


All times are GMT. The time now is 12:25 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11662 seconds with 16 queries