| | Re: maldi compatible silver staining problems
I also tried to use coomassie even colloidal coomassie staining, but unfortunately I have poor results. Therefore, I prefer to use silver staining. I'm also carrying out mass spec analysis. My problem is there are several mass compatible silver staining protocols and I need the best one. For instance, some protocols use formalin in each step but the others use it only in developing step. Some uses only EDTA in stopping step, the others acetic acid. Therefore, I need people who have good mass spec analysis using silver stained spots.