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#1
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| So, I need to figure out how much total protein I should used before digestion with trypsin in order to conduct MS/MS? |
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#2
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| For each protein, ~ 10 pmoles are needed. |
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#3
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| We have got MS/MS done from in-gel-digested peptides from 15 pmoles of BSA in MALDI-TOF-TOF. |
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| digestion , mass spectrometry , needed , protein , rpl4 , solution , total |
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