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How much total protein is needed before digestion of a 78 protein solution?

How much total protein is needed before digestion of a 78 protein solution? - Mass Spectrometry Forum

How much total protein is needed before digestion of a 78 protein solution? - Discuss and post questions about LC-MS, Q-STAR, MALDI-TOF, and other Mass Spectrometry Questions in the Mass Spec Forum.


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Old 08-14-2011, 01:38 AM
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Exclamation How much total protein is needed before digestion of a 78 protein solution?



So, I need to figure out how much total protein I should used before digestion with trypsin in order to conduct MS/MS?
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Old 08-17-2011, 07:49 PM
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Default Re: How much total protein is needed before digestion of a 78 protein solution?

For each protein, ~ 10 pmoles are needed.
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Old 11-27-2011, 05:44 AM
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Default Re: How much total protein is needed before digestion of a 78 protein solution?

We have got MS/MS done from in-gel-digested peptides from 15 pmoles of BSA in MALDI-TOF-TOF.
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digestion , mass spectrometry , needed , protein , rpl4 , solution , total


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