| | Re: In solution
I have had some experience with PNGaseF and glycans. I would run your protein of interest on an SDS-PAGE, perform in-gel Trypsin digestion and extraction. If you have enough sample, I would split your sample in half. a) One half I would clean up with C18 Tips for mass spec analysis. b) The other half of the sample I would incubate with PNGase F, clean up with ZIC-HILIC tips for mass spec analysis.
If you don't have enough sample to split in half I would just do the latter recommendation "b" to identify your glycans.
I hope this helps and Good Luck!
Laboratory Technician at Protea Biosciences, Inc.
Last edited by admin; 03-25-2011 at 06:39 AM.
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