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Missed Trypsin Cleavage

Missed Trypsin Cleavage - Mass Spectrometry Forum

Missed Trypsin Cleavage - Discuss and post questions about LC-MS, Q-STAR, MALDI-TOF, and other Mass Spectrometry Questions in the Mass Spec Forum.


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Old 07-25-2010, 11:08 PM
Pipette Filler
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Default Missed Trypsin Cleavage



I am working on a identification of a specific serine residue and am using an in-gel trypsin protocol, but unable to recover the peptide of interest. I have synthesis the tryspin fragement and am able to identify with MS/MS. A couple questions: 1) Is there ever a time that in-solution trypsin cleavage yields better results, 2) Are there modifications to improve recovery or enhance cleavage?

Thanks,
Don
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Old 08-20-2010, 01:35 PM
Pipette Filler
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Default Re: Missed Trypsin Cleavage

Hi Don,
I have no direct answers for your questions, but just wanna know whether you had tried increase number of miss cleavage and / or MS/MS tolerance, etc... on the Search? YOu might find your peptide fragment then.
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