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Missed Trypsin Cleavage
I am working on a identification of a specific serine residue and am using an in-gel trypsin protocol, but unable to recover the peptide of interest. I have synthesis the tryspin fragement and am able to identify with MS/MS. A couple questions: 1) Is there ever a time that in-solution trypsin cleavage yields better results, 2) Are there modifications to improve recovery or enhance cleavage?
Re: Missed Trypsin Cleavage
I have no direct answers for your questions, but just wanna know whether you had tried increase number of miss cleavage and / or MS/MS tolerance, etc... on the Search? YOu might find your peptide fragment then.
|cleavage , missed , trypsin|
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